P0065

Phospholipase D from Streptomyces chromofuscus

≥50,000 units/mL, buffered aqueous glycerol solution

• 동의어(들): Lecithinase D, Phosphatidylcholine phosphatidohydrolase
• CAS Number: 9001-87-0
• 효소 위원회 번호: 3.1.4.4 (BRENDA, IUBMB)
• EC Number: 232-639-8
• MDL number: MFCD00131897
• UNSPSC 코드: 41106300
• NACRES: NA.77

속성

• 양식: buffered aqueous glycerol solution
• Quality Level: 200
• 특이 활성도: ≥50,000 units/mL
• 분자량: ~60 kDa
• 배송 상태: dry ice
• 저장 온도: −70°C

설명

일반 설명

Phospholipase D (phosphatidylcholine cholinehydrolase, EC 3.1.4.4) is a ubiquitous enzyme with a molecular weight of ~ 50,000Da. It is expressed in a variety of plant tissues, few microorganisms and some mammalian tissues.

애플리케이션

Phospholipase D from Streptomyces chromofuscus has been used to cleave choline-containing phospholipids (PCs).

생화학적/생리학적 작용

Phospholipase D (PLD) catalyses the hydrolysis of phosphatidylcholines (PC) to yield phosphatidic acid (PA) and choline. PC-PLD activity is regulated by hormones, cytokines, neurotransmitters and growth factors. Thus, it is considered to have a role in signal transduction in various cell types. Three vital lipid messengers produced by PLD plays a vital role in various crucial cellular processes and these lipids also have an essential role in downstream triglyceride and phospholipid synthetic pathway. PLD is essential for exocytosis in neurons and endocrine cells. In addition, it also plays a key role in phagocytosis.

Phospholipase D is active on sphingomyelins and lysophospholipids. Phospholipase D hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.

단위 정의

One unit will release 1 μmol of choline from L-α-phosphatidylcholine per hour at pH 8.0 and 30 °C.

물리적 형태

Solution in 100 mM Tris/HCl, pH 8.0, 10% glycerol (v/v), and 0.1% Triton X-100 (w/v).

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)