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Merck
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Key Documents

252859

Sigma-Aldrich

Tris Base

99.8-100.1%,≥99.8%, crystalline, ACS reagent

동의어(들):

Tris(hydroxymethyl)aminomethane, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Trometamol

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About This Item

Linear Formula:
NH2C(CH2OH)3
CAS Number:
Molecular Weight:
121.14
Beilstein:
741883
EC Number:
MDL number:
UNSPSC 코드:
12352104
PubChem Substance ID:
NACRES:
NA.25

product name

Tris(hydroxymethyl)aminomethane, ACS reagent, ≥99.8%

Grade

ACS reagent

Quality Level

분석

≥99.8%
99.8-100.1%

형태

crystalline

기술

affinity chromatography: suitable

불순물

≤0.005% insolubles
≤2% water

색상

white

유용한 pH 범위

7-9

pKa(25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

양이온 미량물

Fe: ≤5 ppm
heavy metals: ≤5 ppm (ICP)

흡수

passes test

응용 분야

clinical research
diagnostic assay manufacturing
microbiology

외래 활성

DNase, none detected
NICKase, none detected
RNase, none detected
protease, none detected

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

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일반 설명

Tris(hydroxymethyl)aminomethane, commonly known as Trometamol, Tris base, or Trizma base, plays a crucial role in diverse research applications as a biological buffer. It is extensively used in the formulation of buffers like Tris-acetate-EDTA (TAE) and Tris-borate-EDTA (TBE), owing to its optimal pKa of 8.1, making it well-suited for maintaining pH within the physiological range (pH 7 -9) applicable to most living organisms. However, caution might be necessary when studying some proteins, as Tris has the potential to interfere with the activity of certain enzymes. Tris base can be employed both independently as a buffer and as a component in mixed buffer formulations, including Tris-EDTA (TE) buffer, TAE buffer, and TBE buffer, among others. Importantly, Tris base is characterized by its purity, essential stability, and relative non-hygroscopic nature.

In laboratory settings, Tris base is indispensable for the preparation of buffers compatible with biological fluids, acting as a standard pH solution. It facilitates various procedures such as lactate dehydrogenase assays, in situ hybridization, and protein extraction from cells. Its versatility extends to applications in cell culture, biochemistry, and molecular biology labs, where it contributes to studies involving cell membrane permeability and buffer preparation.

애플리케이션

Tris(hydroxymethyl)aminomethane has been used:
  • as a buffer solution for lactate dehydrogenase assay, in situ hybridization procedure and protein extraction from cells.
  • as a buffer in flow cell sample preparation for Raman spectroscopy
  • as a wash buffer in Western blotting
  • as a buffer component in decellularization of tissues

특징 및 장점

  • Efficient buffering within the pH range of 7-9 with a pKa of 8.1 (25 °C)
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
  • Can be used in Cell Biology, and Biochemical research

기타 정보

For additional information on our range of Biochemicals, please complete this form.

법적 정보

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

dust mask type N95 (US), Eyeshields, Gloves


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A rhenium tris-carbonyl derivative has been designed to couple infrared and luminescent detection in cells. Both spectroscopies are consistent with one another; they point out the reliability of the present SCoMPI (for Single Core Multimodal Probe for Imaging) for bimodal

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