P0515

Phospholipase D from Arachis hypogaea (peanut)

Type II, lyophilized powder, ≥60 units/mg protein

• 동의어(들): Lecithinase D, Phosphatidylcholine phosphatidohydrolase
• CAS Number: 9001-87-0
• 효소 위원회 번호: 3.1.4.4 (BRENDA, IUBMB)
• EC Number: 232-639-8
• MDL number: MFCD00131897
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: Arachis hypogaea
• Quality Level: 200
• 유형: Type II
• 형태: lyophilized powder
• 특이 활성도: ≥60 units/mg protein
• 구성: Protein, ~30%
• 저장 온도: −20°C

설명

일반 설명

Phospholipase D is a phospholipid hydrolyzing enzyme and an important component of receptor-mediated signal transduction responses and regulated secretion.

Phospholipase D (PLD) is abundantly found in the eukaryotes and prokaryotes. It is present in all mammalian cells. In mammals, PLD is initiated by various hormones, neurotransmitters, growth factors and cytokines.

애플리케이션

Phospholipase D from Arachis hypogaea (peanut) has been used in the preparation of lipase enzyme solution. It has also been used to determine its influence on human neutrophil respiratory function.

Research has shown ADP-ribosylation factor regulation of phospholipase D is important in the release of nascent secretory vesicles from the trans-Golgi network. It has also been used in a study to investigate stimulation of Na⁺-Ca²⁺ exchange activity in canine cardiac sarcolemmal vesicals.

생화학적/생리학적 작용

Hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.

Phospholipase D is involved in conferring drought susceptibility in peanuts, which increases the risk of aflatoxin contamination.

Phospholipase D (PLD) modulates cell growth, secretion and the actin cytoskeleton.

단위 정의

One unit will liberate 1.0 μmol of choline from L-α-phosphatidylcholine (egg yolk) per hr at pH 5.6 at 30 °C.

물리적 형태

Partially purified, lyophilized powder containing buffer salts

분석 메모

Protein determined using biuret, unless otherwise indicated.

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)