추천 제품
검출 방법
colorimetric
관련 질환(들)
neurological disorders
저장 온도
−20°C
유전자 정보
human ... MDH1(4190) , MDH1B(130752) , MDH2(4191)
mouse ... MDH1(17449) , MDH1B(76668) , MDH2(17448)
rat ... MDH1(24551) , MDH1B(316444) , MDH2(81829)
관련 카테고리
일반 설명
Malate dehydrogenase (MDH; EC 1.1.1.37) is an NAD oxidoreductase that catalyzes the NAD/NADH-dependent interconversion of malate and oxalacetate. This reaction is critical to maintain the malate/aspartate shuttle across the mitochondrial membrane and Krebs tricarboxylic acid cycle in the mitochondrial matrix. Increased MDH activity has been observed in neurodegenerative diseases such as Alzheimer′s disease.
애플리케이션
Malate Dehydrogenase Assay Kit has been used in LDH (lactate dehydrogenase) and malate dehydrogenase (MDH1) assays. It has also been used to estimate isocitrate dehydrogenase and succinate dehydrogenase activities.
적합성
Suitable for the detection of Malate Dehydrogenase activity in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.
원리
The Malate Dehydrogenase Assay Kit provides a simple and sensitive procedure for measuring MDH activity in a variety of tissues, cell cultures, and isolated mitochondria. MDH activity is determined by generating a product with absorbance at 450 nm proportional to the enzymatic activity present. One unit of MDH is the amount of enzyme that generates 1.0 μmole of NADH per minute at 37 °C and pH 9.5.
키트 구성품 전용
제품 번호
설명
- MDH Assay Buffer
- MDH Substrate
- MDH Enzyme Mix
- MDH Developer
- NADH Standard
- MDH Positive Control
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1B
Storage Class Code
8A - Combustible corrosive hazardous materials
가장 최신 버전 중 하나를 선택하세요:
시험 성적서(COA)
Lot/Batch Number
Jinliang Wang et al.
Scientific reports, 9(1), 12300-12300 (2019-08-25)
The human mitochondrial heat shock protein 60 (hsp60) is a tetradecameric chaperonin that folds proteins in the mitochondrial matrix. An hsp60 D3G mutation leads to MitCHAP-60, an early onset neurodegenerative disease while hsp60 V72I has been linked to SPG13, a
Two plant-derived aporphinoid alkaloids exert their antifungal activity by disrupting mitochondrial iron-sulfur cluster biosynthesis.
Tripathi S K, et al.
The Journal of Biological Chemistry, jbc-M117 (2017)
Identification of a small molecule inhibitor of 3-phosphoglycerate dehydrogenase to target serine biosynthesis in cancers.
Mullarky E, et al.
Proceedings of the National Academy of Sciences of the USA, 113(7), 1778-1783 (2016)
Anna Antolak et al.
Journal of cellular biochemistry, 118(12), 4323-4330 (2017-04-22)
Drug dependence is an escalating problem worldwide and many efforts are being made to understand the molecular basis of addiction. The morphine model is widely used in these investigations. To date, at least 29 studies exploring the influence of morphine
Edouard Mullarky et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(7), 1778-1783 (2016-02-03)
Cancer cells reprogram their metabolism to promote growth and proliferation. The genetic evidence pointing to the importance of the amino acid serine in tumorigenesis is striking. The gene encoding the enzyme 3-phosphoglycerate dehydrogenase (PHGDH), which catalyzes the first committed step
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.