추천 제품
검출 방법
colorimetric
관련 질환(들)
neurological disorders
저장 온도
−20°C
유전자 정보
human ... MDH1(4190) , MDH1B(130752) , MDH2(4191)
mouse ... MDH1(17449) , MDH1B(76668) , MDH2(17448)
rat ... MDH1(24551) , MDH1B(316444) , MDH2(81829)
일반 설명
Malate dehydrogenase (MDH; EC 1.1.1.37) is an NAD oxidoreductase that catalyzes the NAD/NADH-dependent interconversion of malate and oxalacetate. This reaction is critical to maintain the malate/aspartate shuttle across the mitochondrial membrane and Krebs tricarboxylic acid cycle in the mitochondrial matrix. Increased MDH activity has been observed in neurodegenerative diseases such as Alzheimer′s disease.
애플리케이션
Malate Dehydrogenase Assay Kit has been used in LDH (lactate dehydrogenase) and malate dehydrogenase (MDH1) assays. It has also been used to estimate isocitrate dehydrogenase and succinate dehydrogenase activities.
적합성
Suitable for the detection of Malate Dehydrogenase activity in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.
원리
The Malate Dehydrogenase Assay Kit provides a simple and sensitive procedure for measuring MDH activity in a variety of tissues, cell cultures, and isolated mitochondria. MDH activity is determined by generating a product with absorbance at 450 nm proportional to the enzymatic activity present. One unit of MDH is the amount of enzyme that generates 1.0 μmole of NADH per minute at 37 °C and pH 9.5.
키트 구성품 전용
제품 번호
설명
- MDH Assay Buffer
- MDH Substrate
- MDH Enzyme Mix
- MDH Developer
- NADH Standard
- MDH Positive Control
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1B
Storage Class Code
8A - Combustible corrosive hazardous materials
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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