추천 제품
형태
buffered aqueous glycerol solution
특이 활성도
600-1000 units/mg protein (biuret)
양이온 미량물
NH4+: ≤10 μg/mg protein
외래 활성
Glutamic-Oxalacetic Transaminase ≤0.01%
Glutamic-Pyruvic Transaminase ≤0.01%
배송 상태
wet ice
저장 온도
2-8°C
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일반 설명
Malic dehydrogenase is a mitochondrial isozyme and an important catalyst in the tricarboxylic acid cycle.
Mitochondrial Isozyme
애플리케이션
Malic dehydrogenase has been used in a study to assess the comparative molluscicidal action of Ginko biloba extract on snail hosts. It has also been used in a study to investigate the effect of early feed restriction on metabolic programming and compensatory growth in broiler chickens.
생화학적/생리학적 작용
Eukaryotic cells contain two different isozymes of malate dehydrogenase: mitochondrial (m-MDH) and soluble or cytoplasmic (s-MDH). This product consists of the mitochondrial form.
The enzyme catalyzes the following reaction:Oxaloacetate + β-NADH → L-Malate + β-NAD
The enzyme catalyzes the following reaction:Oxaloacetate + β-NADH → L-Malate + β-NAD
Malic Dehydrogenase can be reversibly inactivated by treatment with pyridoxal 5′-phosphate at 25 °C.
단위 정의
One unit will convert 1.0 μmole of oxalacetate and β-NADH to L-malate and β-NAD per min at pH 7.5 at 25 °C.
물리적 형태
Solution in 50% glycerol containing 0.05 M potassium phosphate buffer, pH 7.5
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
Comparative molluscicidal action of extract of Ginko biloba sarcotesta, arecoline and niclosamide on snail hosts of Schistosoma japonicum
Chen, S., et al.
Pesticide Biochemistry and Physiology, 89, 237-241 (2007)
Nicholas R Moody et al.
Bio-protocol, 11(24), e4264-e4264 (2022-01-29)
Phosphoenolpyruvate carboxylase (PEPC) catalyzes a critical step in carbon metabolism in plants and bacteria, the irreversible reaction between bicarbonate and phosphoenolpyruvate to produce the C4 compound oxaloacetate. This enzyme is particularly important in the context of C4 photosynthesis, where it
S S Chen et al.
The Biochemical journal, 151(2), 297-303 (1975-11-01)
1. Pig heart mitochondrial malate dehydrogenase incubated with pyridoxal 5'-phosphate at pH 8.0 and 25 degrees C gradually loses activity. Such inactivation can be largely reversed by dialysis or by addition of L-lysine or L-cysteine, and can be made permanent
X A Zhan et al.
Poultry science, 86(4), 654-660 (2007-03-21)
The effect of early feed restriction on metabolic programming and compensatory growth was studied in broiler chickens. A total of 480 female 1-d-old broiler birds (Aconred) were randomly allocated to ad libitum and feed-restricted groups, each of which was replicated
Maaike Van Trimpont et al.
Haematologica (2022-08-19)
Asparagine is a non-essential amino acid since it can either be taken up via the diet or synthesized by asparagine synthetase (ASNS). Acute lymphoblastic leukemia (ALL) cells do not or minimally express ASNS which makes them completely dependent on extracellular
프로토콜
Enzymatic Assay of Malic Dehydrogenase (EC 1.1.1.37)
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