LMDH-RO

L-Malate Dehydrogenase (L-MDH)

from pig heart (mitochondrial)

• 효소 위원회 번호: 1.1.1.37 (BRENDA, IUBMB)
• UNSPSC 코드: 12352202

속성

• 생물학적 소스: Porcine heart (mitochondrial)
• Quality Level: 100
• 형태: solution; suspension
• 특이 활성도: ~1200 units/mg protein (At 25 °C with oxaloacetate as the substrate.)
• 포장: pkg of 1 mL (10127248001 solution); pkg of 1 mL (10127256001 suspension); pkg of 5 mL (10127914001suspension)
• 제조업체/상표: Roche
• 기술: activity assay: suitable
• 색상: white
• 최적 pH: 7.4-7.5(reduction of oxaloacetate); 9.2-9.5(oxidation of malate)
• solubility: water: miscible
• 적합성: suitable for Western blot
• NCBI 수납 번호: NM_001244153.1
• UniProt 수납 번호: P00346
• 응용 분야: life science and biopharma
• 외래 활성: Fumarase <0.01%; GIDH 0.001%; GOT 0.001%; GPT 0.00185%; LDH <0.01%; NADH oxidase <0.001%
• 배송 상태: wet ice
• 저장 온도: 2-8°C
• 유전자 정보: Porcine ... MDH2(397039)

설명

일반 설명

L-malate:NAD⁺ oxidoreductase
The L-malate dehydrogenase enzyme is a nuclear gene product that is synthesized with a 24-residue amino-terminal signal peptide. This peptide is proteolytically cleaved during the translocation of the enzyme to the mitochondrial matrix.

애플리케이션

The enzyme L-malate dehydrogenase from pig heart has been used to measure PEPCK (phosphoenolpyruvate carboxykinase) activity. The oxaloacetate, produced by PEPCK, is reduced to malate via the oxidation of NADH, which in turn is measured at 340 nm using a spectrophotometer. The enzyme has also been used to measure oxaloacetate by measuring the reduction in NADH spectroscopically at 340 nm.

생화학적/생리학적 작용

The enzyme L-malate dehydrogenase from pig heart catalyzes the oxidation of L-malate to oxaloacetate. The enzyme is an NAD-dependent mitochondrial dehydrogenase that functions in the tricarboxylic acid cycle. It is a component of the malate-aspartate shuttle that transports reducing equivalents across the inner mitochondrial membrane in the form of malate.

품질

Contaminants: <0.002% GOT, <0.01% fumarase and LDH, each luM each, <0.02% HK, <0.002% PGI

물리적 형태

Solution in 50% glycerol (v/v), pH approximately 7

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

제조 메모

Activator: – phosphate
– arsenate
– Mg²⁺
– Zn²⁺

기타 정보

For life science research only. Not for use in diagnostic procedures.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): No data available
• Flash Point (°C): No data available