추천 제품
생물학적 소스
Porcine heart
양식
ammonium sulfate suspension
특이 활성도
≥100 units/mg protein
분자량
98 kDa ( 49 kDa monomer)
solubility
H2O: soluble 1.0 mg/mL, clear
외래 활성
isocitrate dehydrogenase and aconitase ≤0.01%
malic dehydrogenase ≤0.1%
저장 온도
2-8°C
애플리케이션
Citrate Synthase from porcine heart has been used:
- to inject newt egg for determining its importance in egg activation at fertilization
- in the preparation of reaction mix to determine pyruvate carboxylase enzyme activity
- to examine whether it can induce a [Ca2+] increase and egg activation in unfertilized eggs
생화학적/생리학적 작용
Citrate synthase catalyses the conversion of citrate to acetyl-CoA in the presence of coenzyme-A with the release of H2O and oxaloacetate. The enzyme has a molecular weight of 85 kDa and a pI of 6.1-6.6. It is inhibited by fluoroacetyl-CoA, palmitoyl-CoA, and citroyl-CoA. It is also inhibited when it is acetylated by acetic anhydride or iodinated by iodine.
단위 정의
One unit will form 1.0 μmole of citrate from oxalacetate and acetyl CoA per min at pH 8.0 at 37 °C.
물리적 형태
Suspension in 3.2 M (NH4)2SO4 solution, pH 7.0.
제조 메모
Dissolves in water to form a clear solution at 1 mg/mL concentration.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
이미 열람한 고객
Florian Stengel et al.
Proceedings of the National Academy of Sciences of the United States of America, 107(5), 2007-2012 (2010-02-06)
Small Heat Shock Proteins (sHSPs) are a diverse family of molecular chaperones that prevent protein aggregation by binding clients destabilized during cellular stress. Here we probe the architecture and dynamics of complexes formed between an oligomeric sHSP and client by
Oded Rimon et al.
Antioxidants & redox signaling, 27(15), 1252-1267 (2017-04-11)
A recently discovered group of conditionally disordered chaperones share a very unique feature; they need to lose structure to become active as chaperones. This activation mechanism makes these chaperones particularly suited to respond to protein-unfolding stress conditions, such as oxidative
Xiangjian Gou et al.
Plant biotechnology journal, 20(7), 1417-1431 (2022-04-11)
Single amino acid substitution (SAAS) produces the most common variant of protein function change under physiological conditions. As the number of SAAS events in plants has increased exponentially, an effective prediction tool is required to help identify and distinguish functional
Skylar Xantus Kim et al.
eLife, 7 (2018-07-17)
Anhydrobiotes are rare microbes, plants and animals that tolerate severe water loss. Understanding the molecular basis for their desiccation tolerance may provide novel insights into stress biology and critical tools for engineering drought-tolerant crops. Using the anhydrobiote, budding yeast, we
Alterations in Cytosolic and Mitochondrial [U-13C] Glucose Metabolism in a Chronic Epilepsy Mouse Model
McDonald TS, et al.
eNeuro, 4(1), 266-276 (2017)
문서
Instructions for working with enzymes supplied as ammonium sulfate suspensions
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