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Merck
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주요 문서

MAK333

Sigma-Aldrich

Citrate Assay Kit

sufficient for 100 colorimetric or fluorometric tests

동의어(들):

Citric Acid Test Kit

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About This Item

UNSPSC 코드:
12161503
NACRES:
NA.84

사용

sufficient for 100 colorimetric or fluorometric tests

입력

culture(s)
plasma
tissue
serum
urine

검출 방법

colorimetric
fluorometric

관련 질환(들)

cancer

저장 온도

−20°C

일반 설명

Citrate is an intermediate in the citric acid cycle and is involved in fatty acid synthesis. The Citrate Assay Kit provides a simple, and automation-ready procedure for measuring citrate concentration. Citrate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the citrate concentration in the sample. Linear detection range: 4 to 400 μM citrate for colorimetric assays and 0.5 to 40 μM for fluorimetric assays.

애플리케이션

The Citrate Assay Kit may be used for:
  • Cancer Research
  • Drug Discovery
  • Metabolic Disorders Research

특징 및 장점

Efficient and Precise: Detect Citrate levels ranging from 4 to 400 μM (colorimetric) and 0.5 to 40 μM (fluorimetric) in a 15 minute room temperature reaction, providing both convenience and sensitivity.

Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

적합성

Suitable for citrate determination in biological samples (e.g. plasma, serum, urine, tissue and culture media.)

원리

In this kit, citrate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the citrate concentration in the sample. Linear detection range: 4 to 400 μM citrate for colorimetric assays and 0.5 to 40 μM for fluorimetric assays.

기타 정보

For additional information on our range of Biochemicals, please complete this form.

Storage Class Code

10 - Combustible liquids


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