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Merck
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주요 문서

MAK447

Sigma-Aldrich

Alkaline Phosphatase Assay Kit

Sufficient for 250 colorimetric tests

동의어(들):

ALP Activity Assay Kit, Alkaline Phosphatase Test Kit

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About This Item

UNSPSC 코드:
12161503
NACRES:
NA.84

Quality Level

응용 분야

pharmaceutical

검출 방법

colorimetric

관련 질환(들)

osteoporosis; gastrointestinal diseases; orthopedic diseases; cancer

저장 온도

−20°C

일반 설명

Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in an alkaline environment, resulting in the formation of an organic radical and inorganic phosphate. In mammals, this enzyme is found mainly in the liver and bones. Marked increase in serum ALP levels, a disease known as hyperalkalinephosphatasemia, has been associated with malignant biliary obstruction, primary biliary cirrhosis, primary sclerosing cholangitis, hepatic lymphoma and sarcoidosis.

애플리케이션

The Alkalnine Phosphatase Assay Kit may be used for:
  • Cancer Research
  • Gastroenterology Disease Research
  • Osteoporosis Research

특징 및 장점

Enhanced Detection Range: Detect ALP levels accurately across a wide range, from 2 U/L to 800 U/L, using only 5 μL of sample in a 96-well plate setup, ensuring comprehensive analysis with minimal sample requirement.

Simplified Process: Experience a streamlined process with the addition of only a single working reagent and a short 5 minute assay reaction, reducing complexity and saving valuable time and effort.

Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

적합성

The kit is suitable for the quantitative determination of alkaline phosphatase (ALP) activity in serum, plasma etc.

원리

The Alkaline Phosphatase Assay Kit is designed to measure ALP activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl phosphate that is hydrolyzed by ALP into a yellow colored product (maximal absorbance at A405 nm). The rate of the reaction is directly proportional to the enzyme activity.

기타 정보

For additional information on our range of Biochemicals, please complete this form.

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

표적 기관

Nervous system,Kidney,Liver,Skin

Storage Class Code

8A - Combustible corrosive hazardous materials


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시험 성적서(COA)

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