추천 제품
사용
sufficient for 100 fluorometric tests
검출 방법
fluorometric
관련 질환(들)
endocrinological disorders, diabetes; neurological disorders; cancer
저장 온도
−20°C
관련 카테고리
일반 설명
Protein degradation occurs most commonly through two major mechanisms, lysosomal degradation and proteasome mediated degradation. Lysosomal degradation occurs through proteolytic enzyme activity and is fairly non-specific. Proteasomal degradation is targeted for a specific protein through the ubiquitination of the target protein. The target protein is typically poly-ubiquitinated and activates the 26S proteasome toward proteolysis. The proteasome 26S is the most common form of the proteasome and is an ATP-dependent proteolytic complex, which contains one 20S (700-kDa) core particle structure and two 19S (700-kDa) regulatory caps.
The proteasome is responsible for the recycling of proteins and maintenance of the balance of protein synthesis and degradation. The proteasome is involved with apoptosis, DNA repair, endocytosis, and cell cycle and has been implicated in disease states such as certain types of cancer, diabetes mellitus, and Alzheimer′s disease among others making it the target for drug discovery investigations.
The proteasome is responsible for the recycling of proteins and maintenance of the balance of protein synthesis and degradation. The proteasome is involved with apoptosis, DNA repair, endocytosis, and cell cycle and has been implicated in disease states such as certain types of cancer, diabetes mellitus, and Alzheimer′s disease among others making it the target for drug discovery investigations.
특징 및 장점
Compatible with high-throughput handling systems.
적합성
Suitable for measuring the chymotrypsin-like protease activity associated with the proteasome complex either in cultured cells or cell lysates.
원리
The fluorometric proteasome 20S assay kit is a homogeneous fluorescent assay that measures the chymotrypsin-like protease activity associated with the proteasome complex in cultured cells. This kit uses LLVY-R110 as a fluorogenic indicator for proteasome activities. Cleavage of LLVY-R110 by proteasome generates strongly green fluorescent R110 that is monitored fluorimetrically at 520-530 nm with excitation at 480-500 nm. The assay is robust, and can be readily adapted for high-throughput assays to evaluate the proteasome activities or screen the inhibitors in cultured cells or in solution. The assay can be performed in a convenient 96-well and 384-well fluorescence microtiter-plate format.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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