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Merck
모든 사진(3)

주요 문서

PF0100

Sigma-Aldrich

Protease Fluorescent Detection Kit

high sensitivity assay

동의어(들):

Protease Assay Kit

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About This Item

UNSPSC 코드:
12352202
NACRES:
NA.26

Quality Level

배송 상태

dry ice

저장 온도

−20°C

애플리케이션

Protease Fluorescent Detection Kit has been used:
  • in the detection of proteolytic activity using casein based protease assay
  • for testing the extracellular proteolytic activity of fungal strains
  • for quantifying mitochondrial protease activity
  • for measuring the protease production from soil solution

적합성

Detection limit: approx. 50 ng trypsin

수량

Supplied with sufficient reagents for up to 200 one mL assays.
Supplied with sufficient reagents for up to 200 one mL assays.

The trichloroacetic acid (TCA) component is provided as 6.1 N TCA solution. 0.6 N TCA Solutions may be prepared by a 10-fold dilution of the desired volume of T0699 with ultrapure water. It is not necessary to dilute the entire volume of T0699 for immediate use of the kit.

분석 메모

Tested for detection of all four proteolytic classes (serine, apartic, cysteine and metalloproteinases).

키트 구성품 전용

제품 번호
설명

  • Incubation buffer 25 mL

  • FITC-casein solution 5 mL

  • Assay buffer 200 mL

  • Protease standard (trypsin) 20 μg

  • FITC control 50 mg

  • Trichloroacetic acid (TCA) solution 30 mL

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3

표적 기관

Respiratory system

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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시험 성적서(COA)

Lot/Batch Number

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문서 라이브러리 방문

Qiang Fu et al.
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The somatostatin analog SOM230 has potent radioprophylactic and radiation mitigating properties that are unrelated to cytoprotection but appear to be due to suppression of secretion of pancreatic enzymes into the intestinal lumen. To determine the maximal postirradiation time window for
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Pathogenicity of Pseudomonas syringae is dependent on a type III secretion system, which secretes a suite of virulence effector proteins into the host cytoplasm, and the production of a number of toxins such as coronatine (COR), which is a mimic
Martin H Kunzmann et al.
Molecular bioSystems, 8(11), 3061-3067 (2012-09-20)
α-Alkylidene-γ-butyrolactones are quite common in nature and exhibit a broad spectrum of biological activities. We therefore synthesized a small library of xanthatine inspired α-alkylidene-γ-butyrolactones to screen non-pathogenic and uropathogenic E. coli strains by activity based protein profiling (ABPP). The identified
Joseph S Rom et al.
Virulence, 8(8), 1776-1790 (2017-09-15)
Staphylococcus aureus causes acute and chronic forms of infection, the latter often associated with formation of a biofilm. It has previously been demonstrated that mutation of atl, codY, rot, sarA, and sigB limits biofilm formation in the USA300 strain LAC
Contrasting effects of increased carbon input on boreal SOM decomposition with and without presence of living root system of Pinus sylvestris L.
Linden, A., et al.
Plant and Soil, 377, 145-158 (2014)

프로토콜

The Protease Fluorescent Detection Kit provides ready-to-use reagents for detecting the presence of protease activity. This simple assay to detect protease activity uses casein labeled with fluorescein isothiocyanate (FITC) as the substrate.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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