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L3037

Sigma-Aldrich

Escort™ III Transfection Reagent

Lipid reagent for transfecting sensitive and primary cells

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모든 사진(2)

About This Item

UNSPSC 코드:
41106502
NACRES:
NA.25

Grade

for molecular biology

Quality Level

200

형태

liquid (aqueous solution)

사용

1 mL sufficient for 250-1000 transfections

농도

1 mg/mL

기술

transfection: suitable

저장 온도

2-8°C

일반 설명

Escort™ III is a unique formulation of a proprietary polycationic lipid and a neutral non-transfecting lipid. This liposome-forming compound is used for transfection of nucleic acids into primary cells.

애플리케이션

Suitable for transient and stable transfection of nucleic acids into cultured eukaryotic cells. Use approximately 2-8 μl Escort™ III and 2 μg DNA per 6 cm cell culture plate. Protocol optimization provides very efficient transfection. The following cells have been successfully transfected using Escort™ III:

A549
C2C12 myotubes
Cardiomyocytes (rat)
COS-7
Fibroblasts (rat)
Germ cells (male rat)
Hepatocytes (rat and hamster)
HepG2
HeLa
Jurkat
Keratinocytes (human)
Myoblasts (mouse and quail)
Myocytes (mouse)
NIH3T3
PC-12
Retinal Neurons (rat)
Tracheobronchial cells (sheep)

특징 및 장점

  • Suitable for stable and transient transfection
  • Optimized for a wide variety of primary cells
  • Low toxicity
  • Compatible with both serum and serum-free transfection protocols
  • Ideal for PC-12 cells

성분

Escort™ III formulation:
1 mg/mL total lipid in water

Note the identity of the lipids used in Escort™ III is confidential.

주의사항

Do not freeze.

원리

A stable complex is formed when Escort™ III is mixed with DNA in the absence of serum. The complexes are stable and can be directly added to the cell culture medium, where they fuse with the cell membrane, releasing the DNA into the cytoplasm. Note: complex formation is inhibited by serum, but once stable complexes have formed, the presence of serum is without consequence.

법적 정보

Escort is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

Tomás C O'Riordan et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 292(4), R1613-R1620 (2006-12-16)
The development and application of a methodology for measurement of oxygen within single mammalian cells are presented, which employ novel macromolecular near infrared (NIR) oxygen probes based on new metalloporphyrin dyes. The probes, which display optimal spectral characteristics and sensitivity
Patrick Orth et al.
Molecular biotechnology, 38(2), 137-144 (2008-01-26)
The aim of the present study was to evaluate the efficacy of novel nonviral gene delivery systems in cells of musculoskeletal origin. Primary cultures of lapine skeletal muscle cells, lapine articular chondrocytes, human cells from fibrous dysplasia and cell lines
Inmaculada Navarro-Lérida et al.
Journal of cell science, 117(Pt 9), 1687-1697 (2004-04-13)
Several cell types express inducible nitric oxide synthase (NOS2) in response to exogenous insults such as bacterial lipopolysaccharide (LPS) or proinflammatory cytokines. For instance, muscular cells treated with LPS and interferon gamma (IFN-gamma) respond by increasing the mRNA and protein
Lixian Liu et al.
Frontiers in cell and developmental biology, 9, 634242-634242 (2021-03-12)
The mitogen-inducible gene 6 (MIG6) is an adaptor protein widely expressed in vascular endothelial cells. However, it remains unknown thus far whether it plays a role in angiogenesis. Here, using comprehensive in vitro and in vivo model systems, we unveil
Thomas Iskratsch et al.
The Journal of cell biology, 191(6), 1159-1172 (2010-12-15)
Members of the formin family are important for actin filament nucleation and elongation. We have identified a novel striated muscle-specific splice variant of the formin FHOD3 that introduces a casein kinase 2 (CK2) phosphorylation site. The specific targeting of muscle

문서

Introduction to Cell Transfection

Transfection is the introduction of DNA, RNA, or proteins into eukaryotic cells and is used in research to study and modulate gene expression. Thus, transfection techniques and protocols serve as an analytical tool that facilitates the characterization of genetic functions, protein synthesis, cell growth and development.

How Transfection Works

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

프로토콜

Lipid Transfection Optimization Protocol

Lipid Transfection Optimization Protocol

Escort™ IV Transfection Reagent Protocol

Escort™ IV Transfection Reagent Protocol

Escort™ III Transfection Reagent Protocol

Escort™ III Transfection Reagent Protocol

관련 콘텐츠

Transfection Reagent Selection Guide

Browse our convenient transfection reagent selection guide to match the best reagent for your specific cell line and application needs.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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