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Merck
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주요 문서

SITRAN-RO

Roche

X-tremeGENE siRNA Transfection Reagent

Polymer reagent for delivering siRNA to common cell lines

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About This Item

UNSPSC 코드:
41106502
NACRES:
NA.55

Grade

for molecular biology

Quality Level

사용

sufficient for 2,000 transfections (04476115001)
sufficient for 400 transfections (04476093001)

포장

pkg of 1 mL (04476093001 [1 mg/ml])
pkg of 5 × 1 mL (04476115001 [5 x 1 mg/ml])

제조업체/상표

Roche

농도

1 mg/mL

기술

transfection: suitable

배송 상태

wet ice

저장 온도

2-8°C

일반 설명

Short interfering RNA (siRNA) can be directly introduced into cells by transfection. A lipid-based transfection reagent, such as X-tremeGENE siRNA Transfection reagent, can provide a convenient, reliable, and efficient vehicle for delivering siRNAs into animal cells, enabling the study of cellular and functional consequences of gene knockdown.
This innovative reagent forms a complex with siRNA, as well as with mixtures of siRNA and plasmid DNA (cotransfection), and efficiently delivers the nucleic acids into animal cells to induce gene silencing. Transfection is achieved in just a few steps: mix and incubate diluted transfection reagent with diluted siRNA, then add this complex to cells. Because X-tremeGENE siRNA Transfection Reagent functions exceptionally well in the presence or absence of serum and demonstrates low cytotoxicity, it can be used without media changes. The product is animal-component free.

Contents

Solution filtered through 0.2 μm pore size membrane, supplied in polypropylene tubes.

애플리케이션

X-tremeGENE siRNA Transfection Reagent efficiently delivers short interfering RNA (siRNA) into many commonly used cell types including HeLa, NIH 3T3, HEK-293, CHO-K1, and COS-7, and several hard-to-transfect cell lines, such as HT29, a human adenocarcinoma cell line.

특징 및 장점

  • Knock down gene expression over 90% in many different cell types.
  • Maximize experimental flexibility with a single reagent for siRNA- and cotransfection-based gene-knockdown experiments.
  • Produce meaningful results using a reagent that exhibits low cytotoxicity, ensuring that the cellular effects you observe are due to the transfected siRNA rather than the transfection procedure.
  • Work with or without serum, avoiding medium changes (e.g., to serum-free medium) before or after transfection.;

X-tremeGENE siRNA Transfection Reagent is a proprietary blend of lipids and other components, free of animal products.

품질

Activity assay: X-tremeGENE siRNA Transfection Reagent (1 - 2.5 μl) is combined with siRNA (0.1 - 0.35 μg) that is specific for the HPRT housekeeping gene. The mixture is used to transfect HEK-293 cells (in a monolayer, 30 - 50% confluent) in the presence of 10% fetal bovine serum (FBS). Following transfection, the decrease of HPRT mRNA cells is determined with the LightCycler® Real-Time PCR System. A knockdown efficiency of 70 - 95% is typically observed when mRNA is measured.

Cytotoxicity analysis: HEK-293 cells are exposed to siRNA/X-tremeGENE siRNA Reagent complexes for 72 hours in the presence of serum, without a media change. Cytotoxicity is then tested by analyzing the cells with the WST-1 Cell Proliferation Reagent (Roche).

기타 정보

For life science research only. Not for use in diagnostic procedures.

법적 정보

X-tremeGENE is a trademark of Roche

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point (°F)

does not flash

Flash Point (°C)

does not flash


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문서

Small inhibitory RNAs (siRNAs) have become the focus of interest in many laboratories. For the first time, these molecules offer an easy way to knock down the expression of selected genes in mammalian cells without having to resort to classical gene knockout techniques.

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This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

프로토콜

X-tremeGENE™ siRNA Transfection Reagent Protocol & Troubleshooting

관련 콘텐츠

Browse our convenient transfection reagent selection guide to match the best reagent for your specific cell line and application needs.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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