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Merck
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주요 문서

XTGHP-RO

Roche

X-tremeGENE HP DNA Transfection Reagent

High-performance polymer reagent for transfecting many cell lines

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About This Item

UNSPSC 코드:
41106502
NACRES:
NA.55

가격 및 재고 정보를 현재 이용할 수 없음

Grade

for molecular biology

Quality Level

형태

liquid (aqueous solution)

사용

 mL (suitable for 165 transfections)

포장

pkg of 0.4 mL (06366244001)
pkg of 1.0 mL (06366236001)
pkg of 5 × 1 mL (06366546001)

제조업체/상표

Roche

기술

transfection: suitable

저장 온도

−20°C

유사한 품목 비교

전체 비교 보기

차이점 표시

1 of 4

이 품목
XTG9-ROXTG360-ROSITRAN-RO
grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

form

liquid (aqueous solution)

form

liquid (aqueous solution)

form

liquid (aqueous solution)

form

-

packaging

pkg of 0.4 mL (06366244001), pkg of 5 × 1 mL (06366546001), pkg of 1.0 mL (06366236001)

packaging

pkg of 0.4 mL (06365779001), pkg of 1.0 mL (06365787001), pkg of 5 × 1.0 mL (06365809001)

packaging

pkg of 0.4 mL (08724105001), pkg of 1.0 mL (08724121001), pkg of 5 × 1.0 mL (08724156001)

packaging

pkg of 1 mL (04476093001 [1 mg/ml]), pkg of 5 × 1 mL (04476115001 [5 x 1 mg/ml])

Quality Level

100

Quality Level

100

Quality Level

-

Quality Level

100

일반 설명

X-tremeGENE HP DNA Transfection Reagent is a multi-component reagent that forms a complex with DNA , then transports the complex into animal or insect cells.

특징 및 장점

X-tremeGENE HP DNA Transfection Reagent is a high performance reagent that transfects a broad range of eukaryotic cells, including insect cells and hard-to-transfect cell lines (e.g., Sf9, U-2 OS, MEF, MCF7, Hep G2, PC-3, HeLa, HT-29, HT-1080, K-562, RAW 264.7, Jurkat, PC-12, and HCT 116).

  • Benefit from an easy-to-use non-liposomal reagent that is free of animal-derived components, stable at room temperature, filtered through 0.2 μm pore size membrane, and active in serum-containing medium.
  • Achieve new levels of transfection efficiency in primary cells and tumor cell lines that are not transfected well by other reagents.
  • Generate physiologically relevant data using a reagent with low cytotoxic effects.
  • Increase experimental throughput and enable target evaluation using a simple and consistent protocol.

품질

Each lot of X-tremeGENE HP DNA Transfection Reagent is carefully tested following established quality procedures to ensure that the product is consistently performing according to specifications. During quality testing, cells are transfected with a reporter gene vector DNA using X-tremeGENE HP DNA Transfection Reagent (ratio 3:1 ¼l/¼g DNA). Reporter gene activity is monitored via chemiluminescent detection. Using a standard curve, the total amount of recombinant protein is determined per well in order to meet specification.

물리적 형태

Supplied in 80% ethanol and sterile-filtered through 0.2 μm pore size membrane. Does not contain any ingredients of human or animal origin.Number of Tests: Using the standard procedure, 1 ml of X-tremeGENE HP DNA Transfection Reagent can be used to perform up to 10,000 transfections in 96-well plates.

기타 정보

For life science research only. Not for use in diagnostic procedures.

법적 정보

X-tremeGENE is a trademark of Roche

픽토그램

FlameExclamation mark

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point (°F)

334.4 °F

Flash Point (°C)

168 °C


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    문서

    Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.

    Small inhibitory RNAs (siRNAs) have become the focus of interest in many laboratories. For the first time, these molecules offer an easy way to knock down the expression of selected genes in mammalian cells without having to resort to classical gene knockout techniques.

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    프로토콜

    Lentiviruses represent a powerful tool in research applications to transduce a wide range of cell types.

    관련 콘텐츠

    Browse our convenient transfection reagent selection guide to match the best reagent for your specific cell line and application needs.

    자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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