추천 제품
일반 설명
The MISSION® siRNA Transfection Reagent is for the transfection of siRNA to achieve ≥90% silencing efficiency for transient knockdown of eukaryotic gene expression.
Effective gene silencing and reduced off target effects are achieved using a low siRNA concentration (1nM). The siRNA Transfection Reagent is provided as a sterile solution, and is compatible with serum and antibiotics.
Effective gene silencing and reduced off target effects are achieved using a low siRNA concentration (1nM). The siRNA Transfection Reagent is provided as a sterile solution, and is compatible with serum and antibiotics.
기타 정보
Gene silencing assay: An A549 cell line stably expressing firefly luciferase was transfected in 24-well plates with 8.4 ng of 21-mer Luciferase siRNA using 2 μL of the MISSION® siRNA Transfection Reagent. Experiments were performed in triplicate in presence of serum. Cell lysates were assessed for luciferase expression 48 hours post-transfection.
법적 정보
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
관련 제품
제품 번호
설명
가격
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Warning
유해 및 위험 성명서
Hazard Classifications
Flam. Liq. 3
Storage Class Code
3 - Flammable liquids
WGK
WGK 3
Flash Point (°F)
120.2 °F
Flash Point (°C)
49 °C
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문서
This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.
관련 콘텐츠
Browse our convenient transfection reagent selection guide to match the best reagent for your specific cell line and application needs.
Sigma-Aldrich® Advanced Genomics is the leading provider of gene editing and silencing technologies including CRISPR, Cas9, synthetic guide RNA (sgRNA), and Zinc Finger Nuclease (ZFN).
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