CAPHOS

Calcium Phosphate Transfection Kit

Most cost effective transfection reagent kit for transient and stable transfection of DNA into mammalian cells

• 동의어(들): Gene delivery
• EC Number: 233-140-8
• UNSPSC 코드: 12352200
• NACRES: NA.85

속성

• Grade: for molecular biology
• Quality Level: 200
• 형태: solution
• 사용: kit sufficient for 160 transfections (6 cm dishes); kit sufficient for 400 transfections (3.5 cm dishes); kit sufficient for 80 transfections (10 cm dishes)
• 기술: transfection: suitable
• 배송 상태: dry ice
• 저장 온도: −20°C

설명

일반 설명

Calcium phosphate transfection is a commonly used method for the introduction of DNA into eukaryotic cells. This technique has been used to obtain both transient and stable transfections in a wide variety of cell types.

애플리케이션

Calcium Phosphate Transfection Kit has been used:

• to enable transfection
• to transfect Hek293T cells
• to transfect H29D cells

Suitable for transient and stable transfection of DNA into cultured mammalian cells. The following cells have successfully been transfected using the calcium phosphate method:

BAEC
Bowels melanoma cells
CHO K1
COS-7
Fibroblasts (human embryonic, neo derm)
HEK293
Huh 7
IMR-90
LLC (Lewis Lung Carcinoma)
NIH3T3
PC-12
PCI-13
SH-Sy5Y
SK-Hep-1
T47D

특징 및 장점

• Suitable for transient and stable transfection
• Reproducible for a wide range of cell types
• Widely referenced
• Inexpensive

성분

The Calcium Phosphate Transfection Kit contains:
5 ml 2.5M CaCl2 (C2052)
25 ml 2x HEPES Buffered Saline (H1012)
25 ml molecular biology grade water (W4502)

원리

The procedure is based on slow mixing of HEPES-buffered saline containing sodium phosphate with a CaCl₂ solution containing the DNA. A DNA-calcium phosphate co-precipitate forms, which adheres to the cell surface and is taken up by the cell, presumably by endocytosis.

기타 정보

The protocol was developed for transient transfection of CHO cells using pSV40-CAT plasmid as a reporter gene.

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H319
• 예방조치 성명서: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class Code: 12 - Non Combustible Liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable