G1549

PNGase F from Elizabethkingia meningoseptica

ready-to-use solution, recombinant, expressed in E. coli

• 동의어(들): PNGase F from Elizabethkingia meningoseptica, N-Glycosidase F, PNGase F from Chryseobacterium meningosepticum, PNGase F from Flavobacterium meningosepticum, Peptide N-glycosidase
• CAS Number: 83534-39-8
• 효소 위원회 번호: 3.5.1.52 (BRENDA, IUBMB)
• MDL number: MFCD00131222
• UNSPSC 코드: 12352204

속성

• 재조합: expressed in E. coli
• Quality Level: 200
• 결합: (N-linked)
• Grade: Proteomics Grade
• 형태: ready-to-use solution
• 특이 활성도: ≥1000 U/mg
• 유통기한: ≥1 yr at -20 °C
• 분자량: ~36 kDa
• 배송 상태: wet ice
• 저장 온도: −20°C

설명

애플리케이션

Recombinant PNGase F has been purified by affinity chromatography and dialyzed into a 50% glycerol solution with 10 mM potassium phoosphate pH 7.5 to produce a stable product. The product contains low levels of buffer salts. This highly purified material can be used for preparative deglycosylation or for analytical applications in gel, in solution, or on blot membranes. The enzyme can be removed from preparative operations by utilizing its C-terminal 6x histidine fusion tag. PNGase F from Elizabethkingia meningoseptica has been used in deglycosylation assay in human plasma samples and in deglycosylation of chondroitin sulfate proteoglycan.

Used to deglycosylate protein.

생화학적/생리학적 작용

PNGase F from Elizabethkingia meningoseptica has glycan-binding catalytic domain and a bowl-like domain at the N-terminus. It cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain. It is cost-effectively produced on a large scale in prokaryotic hosts and requires divalent zinc ions for its enzymatic activity.

Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.

단위 정의

One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.

물리적 형태

Supplied as 300 Units/mL enzyme in 50% (v/v) glycerol and 50% (v/v) 20 mM Potassium Phosphate, pH 7.5.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable