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PP0510

Sigma-Aldrich

GlycoProfile™ IV Chemical Deglycosylation Kit

TFMS Deglycosylation System

동의어(들):

Deglycosylation kit

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About This Item

UNSPSC 코드:
12352200
NACRES:
NA.32

Quality Level

200

저장 온도

2-8°C

일반 설명

The GlycoProfile™ IV Kit utilizes trifluoromethane sulfonic acid (TFMS) in a deglycosylation system that completely removes all N- and O-linked glycans while safeguarding the protein or polypeptide structure. TFMS hydrolysis of glycoproteins results in minimal protein degradation while the released glycans are eliminated. The conditions are optimized to deglycosylate simple or low molecular weight glycoproteins in 30 mins without the scavenger. For high molecular weight or complex, non-mammalian glycoproteins, anhydrous anisole is provided as a scavenger to give a high protein yield.

애플리케이션

GlycoProfile™ IV Chemical Deglycosylation Kit has been used in the deglycosylation of human α-L-iduronidase and peritrophic matrix from the gut of the caterpillar, Helicoverpa armigera. It has also been used to estimate the glycosylation of plant-derived recombinant human interleukin-13 (rhIL-13).

특징 및 장점

  • Eliminates both N- and O-linked glycans from proteins
  • This enables sequence analysis of the protein core by mass spectrometry (MS) or DNA-to-protein sequence comparison
  • Eliminates glycans which affect the molecular radius
  • Allows molecular mass determination by electrophoresis
  • Complete deglycosylation in as short as 30 mins for high throughput
  • Quick as it eliminates the need for multiple enzyme reactions
  • Accommodates a broad range of glycoproteins
  • Minimal degradation of the protein core to facilitate reliable MS results

수량

The kit contains sufficient reagents for 10 reactions with a sample size between 1-2 mg of a typical glycoprotein. An RNase B glycoprotein standard is included.

법적 정보

GlycoProfile is a trademark of Sigma-Aldrich Co. LLC

키트 구성품 전용

제품 번호
설명
  • Trifluoromethanesulfonic Acid (TFMS) 5 x 1
  • RNase B Glycoprotein Standard 3 x 1
  • Pyridine solution, 60% 10 mL
  • Bromophenol blue solution, 0.2% .5 mL
  • Anisole, anhydrous 5 x 1
  • Reaction vials 10 ea

신호어

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 1 - STOT SE 3

표적 기관

Eyes,Central nervous system, Respiratory system

Storage Class Code

3 - Flammable liquids

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

A novel platform for biologically active recombinant human interleukin-13 production
David J W, et al.
Plant Biotechnology (2008)

문서

Glycoprotein Deglycosylation

Information about Glycoprotein deglycosylation. The diversity of oligosaccharide structures, both O-linked and N-linked, often results in heterogeneity in the mass and charge of glycoproteins.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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