NGLYF-RO

N-Glycosidase F

PNGase F of Flavobacterium meningosepticum, recombinant from E. coli

• 동의어(들): glycosidase
• 효소 위원회 번호: 3.5.1.52 (BRENDA, IUBMB)
• UNSPSC 코드: 12352200

속성

• 재조합: expressed in E. coli
• Quality Level: 100
• 결합: (N-linked)
• 분석: ≥90% (SDS-PAGE)
• 형태: solution
• 특이 활성도: ~25000 units/mg protein
• 포장: pkg of 0.1 mL (11365169001 [100 U]); pkg of 0.25 mL (11365177001 [250 U])
• 제조업체/상표: Roche
• 최적 pH: 7.0-8.0
• 저장 온도: −20°C

설명

일반 설명

Peptide-N-glycosidase F, Peptide-N⁴-(acetyl-β-glucosaminyl)-asparagine amidase

애플리케이션

Use N-glycosidase F to cleave all types of asparagine-bound N-glycans, provided that the amino group as well as the carboxyl group are present in a peptide linkage, and that the oligosaccharide has the minimum length of the chitobiose core unit. The reaction products are ammonia, aspartic acid (in the peptide chain), and the complete oligosaccharide.
Note: N-Glycosidase F, recombinant is also available as a lyophilizate without glycerol.

단위 정의

One unit is the enzyme activity which hydrolyzes 1 nmol dabsyl fibrin glycopeptide or 0.2 nmol dansyl fetuin glycopeptide within 1 minute at 37 °C at pH 7.8.

물리적 형태

Solution in 50 mM sodium phosphate, 12.5 mM EDTA, 50% glycerol (v/v), pH 7.2

기타 정보

For life science research only. Not for use in diagnostic procedures.

법적 정보

The sale of the Product does not exhaust or grant any rights in third party patents including patents of companies of the F. Hoffmann - La Roche AG group of companies, in particular, for the use of modified antibodies obtained by using the product.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): No data available
• Flash Point (°C): No data available