추천 제품
생물학적 소스
bacterial (Arthrobacter ureafaciens)
Quality Level
양식
solution
특이 활성도
~25 units/mg protein
포장
pkg of 1 U (100 μl)
제조업체/상표
Roche
최적 pH
5.0-5.5
저장 온도
2-8°C
일반 설명
Neuraminidase hydrolyzes terminal N- or O-acylneuraminic acids which are α2,3-, α2,6-, or α2,8-linked (rate: α2,6 > α2,3 > α2,8) to oligosaccharides, polysaccharides, mucopolysaccharides, glycoproteins, and glycolipids. Noteworthy, for the hydrolysis of glycolipids, the presence of a detergent is necessary. Because of the broad substrate specificity, the enzyme is very well suited for the complete removal of sialic acids from glycoconjugates of a wide variety of biological materials.
특이성
Cleaves terminal sialic-acid residues that are α2,3-, α2,6-, or α2,8-linked to Gal, GlcNAc, GalNAc, AcNeu, GlcNeu, oligosaccharides, glycolipids, or glycoproteins. Relative rate of cleavage is α2,6 >α2,3 >α2,8, determined on bonds in tri- and tetrasaccharides.
애플리케이션
- cell surface lectin array analysis.
- hemagglutination assays.
- cell adhesion assay.
For the hydrolysis of glycolipids, the presence of a detergent is necessary. Because of the broad substrate specificity, the enzyme is very well suited for the complete removal of sialic acids from glycoconjugates of a wide variety of biological materials.
Neuraminidase has been used for the:
- detection of the cell surface glycosylations in human anaplastic large cell lymphoma cells
- release of sialic acid from cells
- antibody-overlay lectin microarray
물리적 특성
This product is a mixture of isoenzymes (L, M1, M2 and S) with the following molecular weight values: ~ 52 kDa, 66 kDa and 88 kDa.
물리적 형태
Solution in 10 mM sodium phosphate, 0.1% Micr-O-Protect (w/v), 0.25 mg/ml bovine serum albumin, pH 7
제조 메모
Working concentration: Enzyme/substrate ratio should be in the range of 0.04 U/25-80 μg.
기타 정보
For life science research only. Not for use in diagnostic procedures.
신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Skin Sens. 1
WGK
nwg
Flash Point (°F)
does not flash
Flash Point (°C)
does not flash
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프로토콜
Neuraminidase (Sialidase) Protocol
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