480708

α2-3,6-Neuraminidase, Clostridium perfringens, Recombinant, E. coli

• 동의어(들): α2-3,6-Neuraminidase, Clostridium perfringens, Recombinant, E. coli, Acetylneuraminyl Hydrolase, Sialidase
• CAS Number: 9001-67-6
• 효소 위원회 번호: 3.2.1.18 (BRENDA, IUBMB)
• UNSPSC 코드: 12352202
• NACRES: NA.54

속성

• 재조합: expressed in E. coli
• Quality Level: 100
• 제품 라인: EMPROVE^® EXPERT
• 형태: liquid
• 특이 활성도: ≥10 units/mL; ≥250 units/mg protein
• 제조업체/상표: Calbiochem^®
• 저장 조건: do not freeze
• 외래 활성: N-acetylglucosaminidase, α-fucosidase, α- and β-galactosidase, α- and β-mannosidase, proteases, none detected
• 배송 상태: wet ice
• 저장 온도: 2-8°C

설명

일반 설명

Note: 1 mU = 1 milliunit.

Recombinant, Clostridium perfringens α2-3,6-Neuraminidase expressed in E. coli. Catalyzes the hydrolysis of non-reducing terminal α2,3 and α2,6 unbranched sialic acid residues from complex carbohydrates and glycoproteins. This enzyme does not exhibit activity on α2,8 or branched sialic acids.

애플리케이션

Recommended reaction buffer: 50 mM sodium phosphate buffer, pH 6.0

경고

Toxicity: Harmful (C)

단위 정의

One unit is defined as the amount of enzyme that will catalyze the release of 1 µmol of methylumbelliferone from 2ʹ-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic Acid (Cat. No. 474495) per min at 37°C, pH 5.0.

물리적 형태

In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.

기타 정보

Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
Ohta, Y., et al. 1989. J. Biochem (Tokyo) 106, 1086.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• 픽토그램: GHS05,GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H290 - H315 - H319 - H335
• 예방조치 성명서: P234 - P261 - P264 - P271 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 8B - Non-combustible, corrosive hazardous materials
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable