E9762

Endoglycosidase F1 from Elizabethkingia miricola

recombinant, expressed in E. coli, ≥16 U/mg, buffered aqueous solution

• 동의어(들): Endo F1, Endo-β-N-acetylglucosaminidase F1, Endoglycosidase F1 from Chryseobacterium meningosepticum, Endoglycosidase F1 from Elizabethkingia meningoseptica, Endoglycosidase F1 from Flavobacterium meningosepticum
• 효소 위원회 번호: 3.2.1.96 (BRENDA, IUBMB)
• MDL number: MFCD00146843
• UNSPSC 코드: 12352204
• NACRES: NA.32

속성

• 재조합: expressed in E. coli
• Quality Level: 200
• 결합: (N-linked)
• 형태: buffered aqueous solution
• 특이 활성도: ≥16 U/mg
• 분자량: 32 kDa
• 배송 상태: wet ice
• 저장 온도: 2-8°C

설명

일반 설명

Endoglycosidase F1 from Elizabethkingia miricola is a glycan specific enzyme.

애플리케이션

Endoglycosidase F1 from Elizabethkingia miricola has been used to remove binding of human hemochromatosis protein (HFE) to cation independent mannose-6-phosphate receptor (CI-MPR) and to glycosylate fucosylated N-glycopeptides.

Cleaves asparagine-linked or free oligomannose and hybrid, but not complex, oligosaccharides.

생화학적/생리학적 작용

Endoglycosidase F1 from Elizabethkingia miricola plays an important role in cleavage of glycan structures from the protein by cleaving between the two N-acetylglucosamine residues of the chitobiose core. It mediates high mannose and hybrid oligosaccharides cleavage.

포장

Supplied with 5× Reaction Buffer, 250 mM NaH₂PO₄, pH 5.5.

단위 정의

One unit will release N-linked oligosaccharides from 1 μmole of denatured Ribonuclease B in 1 minute at 37 °C, pH 5.5.

물리적 형태

Aseptically filled solution in 20 mM Tris-HCl pH 7.5

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)