E2264

Endoglycosidase F3 from Elizabethkingia miricola

recombinant, expressed in E. coli, 30 U/mg

• 동의어(들): Elizabethkingia miricola, Endo-β-N-acetylglucosaminidase F3, Endoglycosidase F3 from Elizabethkingia (Chryseobacterium/Flavobacterium) meningosepticum
• 효소 위원회 번호: 3.2.1.96 (BRENDA, IUBMB)
• MDL number: MFCD00146843
• UNSPSC 코드: 12352204
• NACRES: NA.32

속성

• 재조합: expressed in E. coli
• Quality Level: 200
• 결합: (N-linked)
• 양식: solution
• 특이 활성도: 30 U/mg
• 분자량: 32 kDa
• 배송 상태: wet ice
• 저장 온도: 2-8°C

설명

애플리케이션

Endoglycosidase F3 from Elizabethkingia miricola has been used to analyze core fucosylation and tryptic digests of serum proteins.

생화학적/생리학적 작용

Cleaves asparagine-linked biantennary and triantennary complex, oligosaccharides depending on the state of core fucosylation and peptide linkage.

Endoglycosidase F3 belongs to the glycoside hydrolase family 18 (GH18). It has hydrolytic activity. Endoglycosidase F3 glycosylates α-1,6-fucosylated GlcNAc derivative to give natural, core fucosylated complex-type N-glycopeptides.

포장

Supplied with 5× Reaction Buffer, 250 mM sodium acetate, pH 4.5

단위 정의

One unit will release N-linked oligosaccharides from 1 μmole of denatured porcine fibrinogen in 1 minute at 37 °C, pH 4.5.

물리적 형태

Aseptically filled solution in 20 mM Tris-HCl, pH 7.5

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)