E2264

Endoglycosidase F3 from Elizabethkingia miricola

recombinant, expressed in E. coli, 30 U/mg

• 동의어(들): Elizabethkingia miricola, Endo-β-N-acetylglucosaminidase F3, Endoglycosidase F3 from Elizabethkingia (Chryseobacterium/Flavobacterium) meningosepticum
• 효소 위원회 번호: 3.2.1.96 (BRENDA, IUBMB)
• MDL number: MFCD00146843
• UNSPSC 코드: 12352204
• NACRES: NA.32

속성

• 재조합: expressed in E. coli
• Quality Level: 200
• 결합: (N-linked)
• 형태: solution
• 특이 활성도: 30 U/mg
• 분자량: 32 kDa
• 배송 상태: wet ice
• 저장 온도: 2-8°C

설명

애플리케이션

Endoglycosidase F3 from Elizabethkingia miricola has been used to analyze core fucosylation and tryptic digests of serum proteins.

생화학적/생리학적 작용

Endoglycosidase F3 belongs to the glycoside hydrolase family 18 (GH18). It has hydrolytic activity. Endoglycosidase F3 glycosylates α-1,6-fucosylated GlcNAc derivative to give natural, core fucosylated complex-type N-glycopeptides.

Cleaves asparagine-linked biantennary and triantennary complex, oligosaccharides depending on the state of core fucosylation and peptide linkage.

포장

Supplied with 5× Reaction Buffer, 250 mM sodium acetate, pH 4.5

단위 정의

One unit will release N-linked oligosaccharides from 1 μmole of denatured porcine fibrinogen in 1 minute at 37 °C, pH 4.5.

물리적 형태

Aseptically filled solution in 20 mM Tris-HCl, pH 7.5

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)