F6008

Ficin from fig tree latex

lyophilized powder

• 동의어(들): Debricin, Ficain, higueroxyl delabarre
• CAS Number: 9001-33-6
• 효소 위원회 번호: 3.4.22.3 (BRENDA, IUBMB)
• EC Number: 232-599-1
• MDL number: MFCD00081597
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: fig tree (latex)
• Quality Level: 300
• 형태: lyophilized powder
• 특이 활성도: ≥1.0 units/mg protein
• 분자량: 23.8 kDa
• 구성: Protein, 80-100% biuret
• 저장 온도: −20°C

설명

일반 설명

Extinction Coefficient: E^1% = 21.0 (280 nm)
pI: 9.0

애플리케이션

Ficin from fig tree latex has been used:

• in the proteolysis of tendon collagen
• in the digestion of Chlorophyta Ulva rigida
• to investigate it effect on protease-activated receptors 2 and 4 activation to induce itch in skin

생화학적/생리학적 작용

Ficin is classified as a thiol protease. It contains a single reactive cysteine at its active site. The amino acid homology of the active site is similar to that of papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported K_m for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. Ficin is inhibited by iodoacetamide, iodoacetic acid, N-ethylmaleimide, mercuric chloride, DFP (diisopropyl fluorophosphate), TLCK (Na-p-Tosyl-lysine chloromethyl ketone), and TPCK (N-Tosyl-L-phenylalanine chloromethyl ketone). Ficin can be used to generate high yielding F (ab′)₂ fragments from mouse IgG1.

Ficin is effective in disrupting the staphylococcal biofilms making it accessible antibiotics exposure.

단위 정의

One unit will produce a ΔA₂₈₀ of 1.0 per min at pH 7.0 at 37°C when measuring TCA soluble products from casein in a final volume of 10 ml (1 cm light path).

물리적 형태

Lyophilized powder containing L-cysteine and trace EDTA

제조 메모

This enzyme is soluble in water (10 mg/ml), yielding a clear solution.

안전성 정보

• 픽토그램: GHS08,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H315,H319,H334,H335
• 예방조치 성명서: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• 개인 보호 장비: dust mask type N95 (US), Eyeshields, Faceshields, Gloves