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Merck
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Key Documents

10108014001

Roche

Papain

from Carica papaya

동의어(들):

cystein protease, papain

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About This Item

효소 위원회 번호:
UNSPSC 코드:
12352204

생물학적 소스

papaya

Quality Level

형태

suspension

특이 활성도

30 U/mg
~30 units/mg protein (At 25 °C with BAEE as the substrate.)

분자량

23 kDa

포장

pkg of 10 mL (100 mg)

제조업체/상표

Roche

농도

10-100 mg/mL

최적 pH

6.0-7.0

배송 상태

wet ice

일반 설명

Papain is a cysteine protease of the papain family. It is a single chain protein. The molecular weight of papain is 23 kDa. Papain is composed of two structural domains and a cleft between them, which consists of the active site.

애플리케이션

Papain has been used in the digestion of various brain cells.
Use Papain for the complete proteolytic cleavage of proteins, limited hydrolysis of native immunoglobulins, and tissue dissociation (together with collagenase, esterase, and trypsin). The enzyme solubilizes integral membrane proteins and produces glycopeptides from purified proteoglycans. The addition of cysteine (approximately 0.5% [w/v]) is essential for enzyme activity.

생화학적/생리학적 작용

Papain exhibits proteolytic activity against amide links, amino acid esters, peptides, proteins. It mainly cleaves peptide bonds containing amino acids such as lysine, arginine and residues succeeding phenylalanine. It can catalyze several reactions, such as hydrolysis, transferase action, specificity and acyl-enzyme intermediate.

특징 및 장점

Inhibitors: SH-blocking reagents, iodoacetic acid, iodoacetamide, TPCK, TLCK, leupeptin, a2-macroglobulin, E-64, PMSF, and antipain, Hg2+, and other heavy metals

물리적 형태

Suspension, crystalline, nonsterile

제조 메모

Working concentration: 0.05 to 0.5 mg/ml

저장 및 안정성

Store at 2 to 8 °C. (a decrease in activity of 20% may occur within 6 months)

기타 정보

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point (°F)

does not flash

Flash Point (°C)

does not flash


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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