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Merck
모든 사진(1)

Key Documents

10108057001

Roche

Pepsin

lyophilized (salt-free), ~2500 units/mg protein (At 37 °C with hemoglobin as the substrate. One unit is the enzyme activity which liberates the amount of Tyr producing an increase in the absorbance of 0.001/minute at 280 nm.)

동의어(들):

pepsin

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About This Item

효소 위원회 번호:
UNSPSC 코드:
12352204

생물학적 소스

pig gastric mucosa

Quality Level

형태

lyophilized (salt-free)

특이 활성도

~2500 units/mg protein

포장

pkg of 1 g

제조업체/상표

Roche

최적 pH

1.8-2.2

배송 상태

wet ice

저장 온도

2-8°C

일반 설명

Pepsin is an aspartic endoproteinase isolated from pig gastric mucosa. It is mainly used for the unspecific hydrolysis of proteins and peptides in acidic media. It also provides a limited hydrolysis of native immunoglobulins to yield biologically active fragments.

특이성

Aspartic endopeptidase with relatively broad specificity. Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1′ positions.

애플리케이션

Pepsin has been used for the digestion of liver tissue obtained from rat.

제조 메모

Working concentration: 0.05 to 0.5 mg/ml
Storage conditions (working solution): A pepsin solution is stable at 2 to 8 °C, at least for one week, at neutral pH and under germfree conditions. Frozen aliquots of the enzyme solution are expected to be more stable.

A stock solution in 10 mM HCl (5-10 mg/ml) should be stable for at least one week at 2 to 8 °C, too. Storage light protected, tightly closed.

A short (2 minutes) incubation at 40 °C has negligible effect on the activity of lyophilized pepsin or pepsin in solution.

분석 메모

At 37 °C with hemoglobin as the substrate. One unit is the enzyme activity which liberates the amount of Tyr producing an increase in the absorbance of 0.001/minute at 280 nm.

기타 정보

For life science research only. Not for use in diagnostic procedures.

픽토그램

Exclamation markHealth hazard

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

표적 기관

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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