F4125

Ficin from fig tree latex

saline suspension, ≥1.0 units/mg protein (biuret)

• 동의어(들): Debricin, Ficain, higueroxyl delabarre
• CAS Number: 9001-33-6
• 효소 위원회 번호: 3.4.22.3 (BRENDA, IUBMB)
• MDL number: MFCD00081597
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: fig tree (latex)
• 형태: saline suspension
• 특이 활성도: ≥1.0 units/mg protein (biuret)
• 분자량: 23.8 kDa
• 저장 온도: 2-8°C

설명

일반 설명

Ficin from fig tree latex exists in multiple isoforms. The isoforms are prone to autolysis at varying temperature and tend to degrade during long term storage. It is a sulfhydryl protease with eight cysteine residues and is stabilized by three disulfide bridges.

Extinction Coefficient: E^1% = 21.0 (280 nm)
pI: 9.0

애플리케이션

Ficin from fig tree latex has been used:

• in the electron paramagnetic resonance (EPR) and steady-kinetic measurements for assessing its peroxidase functionality
• for the digestion of eye tissue sections prior to immunostaining and immunofluorescence

생화학적/생리학적 작용

Ficin is classified as a thiol protease. It contains a single reactive cysteine at its active site. The amino acid homology of the active site is similar to that of papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported K_m for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. Ficin is inhibited by iodoacetamide, iodoacetic acid, N-ethylmaleimide, mercuric chloride, DFP (diisopropyl fluorophosphate), TLCK (Na-p-Tosyl-lysine chloromethyl ketone), and TPCK (N-Tosyl-L-phenylalanine chloromethyl ketone). Ficin can be used to generate high yielding F (ab′)₂ fragments from mouse IgG1.

단위 정의

One unit will produce a ΔA₂₈₀ of 1.0 per min at pH 7.0 at 37 °C when measuring TCA soluble products from casein in a final volume of 10 mL (1 cm light path).

물리적 형태

Suspension in 2.0 M NaCl and 0.03 M cysteine, pH 5.0

제조 메모

2× Crystallized

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type ABEK (EN14387) respirator filter