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Merck
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Key Documents

추가 자료

E4913

Sigma-Aldrich

Plant/Fungi RNA Purification Kit

greener alternative

sufficient for 50 purifications

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모든 사진(7)

About This Item

UNSPSC 코드:
41105501
NACRES:
NA.52

사용

sufficient for 50 purifications

환경친화적 대안 제품 특성

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

환경친화적 대안 카테고리

, Aligned

일반 설명

Norgen Biotek′s Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA, including viral RNA, from a wide range of plant and filamentous fungal species. Total RNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit. All sizes of RNA are purified, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The procedure is rapid and convenient, as it does not rely on the use of liquid nitrogen in order to homogenize the samples.

애플리케이션

For the rapid purification of total RNA (including microRNA) from plants and fungi.
Purified RNA is of the highest quality and can be used in a number of downstream applications, including:
  • qRT-PCR
  • Reverse transcription PCR
  • Northern blotting
  • RNAse protection
  • Primer extension
  • Expression array assays
  • Next Generation Sequencing

특징 및 장점

  • Isolate RNA from a wide range of plant and filamentous fungi samples.
  • Robust lysis buffer is suitable for challenging samples such as pine needle or grape leaf.
  • Isolate a diversity of RNA species from large mRNA, microRNA, and viral RNA.
  • Purification of high yield and quality RNA.
  • No phenol/chloroform extraction required.
  • Liquid nitrogen is not required for homogenization

기타 정보

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

관련 제품

제품 번호
설명
가격

픽토그램

Exclamation markEnvironment
GHS07,GHS09

신호어

Warning

유해 및 위험 성명서

H302 + H332,H315,H319,H410

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Yuan Song et al.
Biochemical and biophysical research communications, 396(2), 187-192 (2010-03-25)
DNA methyltransferase2 (DNMT2) is always deemed to be enigmatic, because it contains highly conserved DNA methyltransferase motifs but lacks the DNA methylation catalytic capability. Here we show that Arabidopsis DNA methyltransferase2 (AtDNMT2) is localized in nucleus and associates with histone
Michaela Novodvorska et al.
BMC genomics, 14, 246-246 (2013-04-13)
Genome-wide analysis was performed to assess the transcriptional landscape of germinating A. niger conidia using both next generation RNA-sequencing and GeneChips. The metabolism of storage compounds during conidial germination was also examined and compared to the transcript levels from associated
Jun-Zhi Wen et al.
PloS one, 9(2), e88179-e88179 (2014-02-12)
MicroRNAs (miRNAs) are important regulators of many cellular processes and exist in a wide range of eukaryotes. High-throughput sequencing is a mainstream method of miRNA identification through which it is possible to obtain the complete small RNA profile of an
Hila Yaffe et al.
BMC research notes, 5, 45-45 (2012-01-21)
Rapid RNA extraction is commonly performed with commercial kits, which are very expensive and can involve toxic reagents. Most of these kits can be used with healthy plant tissues, but do not produce consistently high-quality RNA from necrotic fungus-infected tissues
Kobi Buxdorf et al.
Plant molecular biology, 84(1-2), 37-47 (2013-08-15)
The cuticle plays an important role in plant interactions with pathogens and with their surroundings. The cuticle acts as both a physical barrier against physical stresses and pathogens and a chemical deterrent and activator of the plant defense response. Cuticle
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프로토콜

Plant RNA/DNA Purification Kit

The Plant RNA/DNA Purification Kit provides a rapid method for the isolation and purification of total RNA and DNA simultaneously from a single sample of plants.

Plant/Fungi DNA Isolation Kit Protocol

The Plant/Fungi DNA Isolation Kit provides a rapid method for the isolation and purification of total DNA from a wide range of plant and fungi species.

Plant/Fungi DNA Isolation Kit Protocol

The Plant/Fungi DNA Isolation Kit provides a rapid method for the isolation and purification of total DNA from a wide range of plant and fungi species.

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