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Key Documents

DMN10

Sigma-Aldrich

GenElute Direct mRNA Miniprep Kits

sufficient for 10 purifications

동의어(들):

GenElute Direct mRNA Miniprep Kit, GenElute mRNA Kit, Gen Elute

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About This Item

UNSPSC 코드:
41105501
NACRES:
NA.52

사용

sufficient for 10 purifications

Quality Level

기술

RNA purification: suitable

저장 온도

15-25°C

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일반 설명

The GenElute Direct mRNA Miniprep kit provides a convenient format to isolate polyadenylated mRNA directly from mammalian cells and tissues. The direct mRNA isolation procedure is based on that of Badley. Up to 107 mammalian cells or 40 mg tissue are lysed and homogenized, either with the filtration columns provided or with
a mechanical homogenizer. RNase is eliminated during a 10 minute proteinase K digestion. Sodium chloride is added, and polyadenylated RNA is captured on oligo(dT) polystyrene beads during a 10 minute incubation. For further enrichment, RNA may be released from the beads into fresh lysis solution and recaptured with the original beads. After 3 washes in a spin column, purified mRNA is eluted in 100 μl of 10 mM Tris-HCl, pH 7.4.

애플리케이션

The GenElute Direct Kit mRNA kit provides convenient procedures for isolating polyadenylated mRNA from previously prepared total RNA or directly from mammalian cells and tissues.

특징 및 장점

  • mRNA captured on oligo(dT) polystyrene beads in 10 minutes, with no mixing or rocking (Fig. 1)
  • Poly (A)+ mRNA isolated from total RNA in 40 minutes (Fig. 2) or 60 minutes directly from cells and tissues (Fig. 3)
  • Oligo(dT) polystyrene beads require fewer wash steps

기타 정보

Use for isolating mRNA directly from mammalian cells or tissues.
For additional information, please see www.sigma-aldrich.com/mrna.

법적 정보

GenElute is a trademark of Sigma-Aldrich Co. LLC

키트 구성품 전용

제품 번호
설명

  • Elution solution 1.5 mL

  • Filtration columns with tubes 10 ea

  • Lysis solution 20 mL

  • 5 M NaCl 1.5 mL

  • Oligo(dT)-polystyrene beads .3 mL

  • Proteinase K 5 mg

  • 40% Glycerol solution .6 mL

  • Spin columns with tubes 10 ea

  • Collection tube 10 ea

모두 보기 (9)

픽토그램

Health hazardCorrosionExclamation mark

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

표적 기관

Respiratory system

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

Brahmchetna Singh et al.
Molecular cancer, 6, 82-82 (2007-12-26)
Retinoids are potent growth inhibitory and differentiating agents in a variety of cancer cell types. We have shown that retinoids induce growth arrest in all pancreatic cancer cell lines studied, regardless of their p53 and differentiation status. However, the mechanism

프로토콜

Method for reverse transcription of RNA into DNA. Uses a premixed reagent that contains reverse transcriptase, dNTPs, primers, RNase inhibitor and buffer. Fast generation of cDNA.

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