추천 제품
사용
sufficient for 70 purifications
Quality Level
환경친화적 대안 제품 특성
Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
기술
DNA purification: suitable
환경친화적 대안 카테고리
, Aligned
저장 온도
15-25°C
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일반 설명
Plant tissue is disrupted by grinding in liquid nitrogen, and DNA is released with detergent and chaotrope. Proteins, polysaccharides, and cell debris are eliminated with a 10 minute precipitation procedure followed by centrifugation through a filtration column, included in the kit. The genomic DNA is purified further by a silica bind-wash-elute procedure in microcentrifuge spin columns.
The purified genomic DNA is ready for immediate use in downstream applications such as PCR, restriction digestion, cloning and Southern blots.
애플리케이션
- PCR
- restriction endonuclease digestion
- cloning
- Southern blots
- PCR
- restriction endonuclease digestion
- cloning
- Southern blots
GenElute™ Plant Genomic DNA Miniprep Kit has been used:
- in the purification of genomic DNA isolated from neem tissues
- to extract genomic DNA from embryos
- to isolate DNA from plant samples
특징 및 장점
- Starting material: Up to 100 mg of plant tissue
- Expected yield: Up to 20 μg
- Elution volume: 100 - 200 μl
- Time required: < 40 min
- RNase treatment required: No
원리
기타 정보
법적 정보
관련 제품
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1C
보충제 위험성
Storage Class Code
8A - Combustible corrosive hazardous materials
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
가장 최신 버전 중 하나를 선택하세요:
이미 열람한 고객
문서
The Extract-N-Amp™ kits are designed to rapidly extract and amplify genomic DNA. The plant tissue version of these kits has been optimized to amplify without concern over plant inhibitors. This technical document will discuss the versions of this kit that are available and help you find the best kit suited for your needs.
The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.
프로토콜
This protocol describes a simple and convenient procedure to isolate pure DNA from a variety of plant species using the GenElute Plant Genomic DNA Miniprep Kit.
관련 콘텐츠
Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.