R4642

Ribonuclease A from bovine pancreas

(Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)

• 동의어(들): Pancreatic Ribonuclease, RNAsea, RNase A, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase
• CAS Number: 9001-99-4
• 효소 위원회 번호: 3.1.27.5 (BRENDA, IUBMB)
• MDL number: MFCD00132181
• UNSPSC 코드: 12352204
• NACRES: NA.53

속성

• 생물학적 소스: bovine pancreas
• Quality Level: 200
• Grade: for molecular biology
• 형태: (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)
• 분자량: 13.7 kDa; ~13,700
• 농도: 20-40 mg/mL
• 적합성: suitable for
• 외래 활성: Endonuclease and exonuclease, none detected; NICKase and DNase, none detected
• 저장 온도: −20°C
• InChI: 1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
• InChI key: CQOVPNPJLQNMDC-UHFFFAOYSA-N

설명

일반 설명

RNase A is an endoribonuclease that attacks at the 3′OHphosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA.

RNase A, Ribonuclease A, is an endoribonuclease that cleaves the phosphodiester bonds of single strand RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end (For example pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG). The highest activity is exhibited with single stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges. In contrast to RNase B, it is not a glycoprotein. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase A can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts. RNAse is inhibited in the presence of heavy metal ions. RNase is also inhibited competitively by DNA.

애플리케이션

• RNase A is used to remove RNA from DNA plasmid and genomic DNA preparations and protein samples.
• RNase A is also used in RNA sequence analysis and protection assays.
• RNase A has been used as a tool for computer-aided drug design.
• RNase A supports the analysis of RNA sequences.
• RNase A hydrolyze RNA contained in protein samples.
• Purification of DNA is supported by RNase A.

Suitable for:

• RNase protection assays
• Removal of unspecifically bound RNA
• Analysis of RNA sequences
• Hydrolysis of RNA contained in protein samples
• Plasmid DNA purification

특징 및 장점

Our highly stable Ribonuclease A, RNase A, is suitable for removal of RNA, RNA sequencing, and DNA purification.

성분

RNase A is supplied as a solution of 50% glycerol containing 10 mM Tris-HCl (pH 8.0).

단위 정의

A major application for RNase A is the removal of RNA from preparations of plasmid DNA. For this application, DNase free RNase A is used at a final concentration of 10 ug/mL.

Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity. If an RNase A solution is heated at a neutral pH, precipitation will occur. When heated at a lower pH, some precipitation may occur because of protein impurities that are present.

분석 메모

Protein determined by E.

기타 정보

Activators of RNase A include potassium and sodium salts. RNase A can be inhibited by alkylation of His12 or His119.

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable