D7291

Deoxyribonuclease I RNase-free solution from bovine pancreas

• 동의어(들): DNase I, Deoxyribonuclease I, pancreatic DNase I, pancreatic deoxyribonuclease I
• CAS Number: 9003-98-9
• 효소 위원회 번호: 3.1.21.1 (BRENDA, IUBMB)
• EC Number: 232-667-0
• MDL number: MFCD00130918
• UNSPSC 코드: 12352204
• NACRES: NA.53

속성

• 생물학적 소스: bovine pancreas
• Quality Level: 200
• Grade: for molecular biology
• 형태: buffered aqueous glycerol solution
• 특이 활성도: 10000 units/mg protein
• 분자량: 29.1 kDa
• 농도: 5—15 mg protein/mL
• 기술: DNA extraction: suitable
• 적합성: suitable for molecular biology
• UniProt 수납 번호: P00639
• 응용 분야: cell analysis
• 외래 활성: RNase, none detected
• 저장 온도: −20°C
• 유전자 정보: cow ... DNASE1(282217)

설명

일반 설명

Deoxyribonuclease I digests single- and double-stranded DNA to a mixture of mono and oligonucleotides carrying 5′phosphates and 3′OH termini. This catalytic activity is divalent ion-dependent. In the presence of Mg²⁺, DNase I hydrolyzes each strand of double-stranded DNA randomly and independently. In the presence of Mn²⁺, both strands can be cleaved.

애플리케이션

Deoxyribonuclease I RNase-free solution from bovine pancreas has been used to digest DNA from various samples.

Used in molecular biology applications for removing DNA during RNA purification, for preparing DNA for nick translation, and for DNA-protein interaction analysis by footprinting methods.

성분

DNase I is provided in a solution of 50% (v/v) glycerol, 20 mM sodium acetate (pH 6.5), 5 mM CaCl2, and 0.1 mM PMSF.

단위 정의

One unit will cause a ΔA₂₆₀ of 0.001 per min per mL reaction mixture using calf thymus DNA as substrate.

기타 정보

RNA treated with this DNase I should not be used to generate a cDNA library or used for RT-PCR. For these sensitive applications, it is recommended to use DNase I, Amplification Grade , Catalog Number AMP-D1.

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)