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일반 설명
The Extract-N-Amp Plant PCR Kits contain all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by PCR. A novel Extraction Solution eliminates the need for conventional freezing of plant tissues with liquid nitrogen, mechanical disruption, organic extraction, column purification, or precipitation of DNA. The kit also includes a PCR ReadyMix™, especially formulated for amplification directly from extract.
Genomic DNA is extracted from 0.5 to 0.7 cm plant leaf disks that have been cut with a standard paper punch and simply incubated in Extraction Solution at 95 oC for 10 minutes. An equal volume of Dilution Solution is added to the extract to neutralize inhibitory substances prior to PCR. A portion of the DNA extract is then added to a PCR reaction containing primers and the Extract-N-Amp PCR ReadyMix, included in the kit.
Genomic DNA is extracted from 0.5 to 0.7 cm plant leaf disks that have been cut with a standard paper punch and simply incubated in Extraction Solution at 95 oC for 10 minutes. An equal volume of Dilution Solution is added to the extract to neutralize inhibitory substances prior to PCR. A portion of the DNA extract is then added to a PCR reaction containing primers and the Extract-N-Amp PCR ReadyMix, included in the kit.
The Extract-N-Amp™ Plant PCR Kits are intended for direct PCR (polymerase chain reaction). This kit contains all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by PCR. It is a novel Extraction Solution that eliminates the need for conventional freezing of plant tissues.
애플리케이션
Extract-N-Amp™ Plant PCR Kit has been used for genomic DNA isolation from plants and the fungal mycelium. It has also been used in polymerase chain reaction (PCR).
Extract-N-Amp™ Plant PCR Kit is suitable for use in genotyping.
It has been used forDNA extraction from fresh mushrooms.
It has been used forDNA extraction from fresh mushrooms.
특징 및 장점
- Single-step extraction of plant genomic DNA for PCR in less than 15 minutes
- A PCR ReadyMix™, specially formulated for amplification directly from extract
- Hot Start antibody for highly specific PCR amplification of genomic DNA
- Extract stable at 4 °C for at least 6 months
- Plant genomic DNA can be extracted for PCR in under 15 minutes using a method.
- No freezing, mechanical disruption, organic extraction, column purification or precipitation is necessary
- A specially formulated PCR ReadyMix is provided for use with the extracted DNA
- The Hot Start antibody ensures highly specific PCR amplification of genomic DNA
- Suitable for high-throughput plant genetic analysis.
- Lengthy enzymatic digestions are not required, saving time and effort.
- The extracted DNA remains stable at 4 °C for atleast 6 months.
성분
Extract-N-Amp™ Plant PCR Kits contain Dilution solution, Extraction solution, and Extract-N-Amp PCR Reaction Mix (2X) (buffer, salts, dNTPs, Taq polymerase, and JumpStart Taq antibody)
기타 정보
For additional information, please see www.sigma-aldrich.com/extract-n-amp.
The Extract-N-Amp Plant PCR Kits are for laboratory use only. Not for drug, household, or other uses.
법적 정보
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
Storage Class Code
10 - Combustible liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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문서
The Extract-N-Amp™ kits are designed to rapidly extract and amplify genomic DNA. The plant tissue version of these kits has been optimized to amplify without concern over plant inhibitors. This technical document will discuss the versions of this kit that are available and help you find the best kit suited for your needs.
The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.
프로토콜
Extract-N-Amp™ Plant PCR Kits Protocol
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