추천 제품
형태
lyophilized powder
특이 활성도
8-20 units/mg solid
분자량
71 kDa by SDS-PAGE
83 kDa by gel filtration
저장 온도
−20°C
일반 설명
Research area: Cell signaling. Choline oxidaseis a flavoprotein and belongs to the glucose methanol choline(GMC)-oxidoreductase family.
애플리케이션
Choline Oxidase from Arthrobacter globiformis has been used:
- for enzymatic determination of choline (Cho) and phosphocholine (P-Cho)
- in sphingomyelin assay to determine the concentration of sphingomyelin by measuring free choline
- in enzyme coating for the fully-assembled recording device for electrochemical measurements
Choline oxidase from Sigma has been used in the enzymatic determination of choline in milk using a flow injection analysis (FIA) system with potentiometric detection.Choline oxidase from Arthrobacterglobiformis is suitable for the preparation of amperometric biosensors.
생화학적/생리학적 작용
Choline oxidase catalyzes the four-electron oxidation of choline to glycine-betaine, with betaine-aldehyde as intermediate and molecular oxygen as primary electron acceptor. The enzyme can also accept betaine-aldehyde as a substrate. This allows the investigation of the reaction mechanism for the conversion of choline to the aldehyde intermediate and of betaine-aldehyde to glycine-betaine. The enzyme is a flavoprotein with a molecular weight of approximately 83,000 Da according to gel filtration or approximately 71,000 Da according to SDS gel electrophoresis. The optimum pH is found to be around pH 7.5 and the isoelectric point (pI) around pH 4.5.
단위 정의
One unit will form 1 μmole of H2O2 with oxidation of 1 μmole of choline to betaine aldehyde per min at pH 8.0 at 37 °C. Note: During the conversion of choline to betaine by choline oxidase, 2 μmoles of H2O2 are produced for every μmole of choline.
신호어
Danger
유해 및 위험 성명서
예방조치 성명서
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
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Disney AA, et al.
Journal of Neuroscience Methods, 255, 29-37 (2015)
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Nuccio ML, et al.
Metabolic engineering, 2(4), 300-311 (2000)
Shihua Hou et al.
Biosensors & bioelectronics, 33(1), 44-49 (2012-01-11)
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Choline oxidase catalyzes the four-electron oxidation of choline to glycine-betaine, with betaine-aldehyde as intermediate and molecular oxygen as primary electron acceptor. The enzyme is capable of accepting betaine-aldehyde as a substrate, allowing the investigation of the reaction mechanism for both
Shengyuan Deng et al.
Biosensors & bioelectronics, 26(11), 4552-4558 (2011-06-10)
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