콘텐츠로 건너뛰기
Merck
모든 사진(7)

주요 문서

C2888

Sigma-Aldrich

Acetylcholinesterase from Electrophorus electricus (electric eel)

Type V-S, lyophilized powder, ≥1,000 units/mg protein

동의어(들):

AChE, Acetylcholine acetylhydrolase, Cholinesterase, Acetyl

로그인조직 및 계약 가격 보기


About This Item

CAS Number:
효소 위원회 번호:
EC Number:
MDL number:
UNSPSC 코드:
12352204
NACRES:
NA.32

생물학적 소스

Electrophorus electricus

Quality Level

유형

Type V-S

형태

lyophilized powder

특이 활성도

≥1,000 units/mg protein

분자량

280 kDa

구성

Protein, ≥45% biuret

pI 

~5.3

solubility

Tris buffer: 1 mg/mL (0.02 M Tris buffer, pH 7.5)

저장 온도

−20°C

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

Molecular Weight: 280 kDa
Isoelectric Point: 5.5
Extinction Coefficient: E1% = 18.0 (280 nm)

Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.

애플리케이션

Acetylcholinesterase from Electrophorus electricus (electric eel) has been used:
  • in acetylcholinesterase activity assay
  • to study its effects on Xenopus embryonic hippocampal cells
  • to study the effect of acetylcholine inhibitors on development and plasticity

생화학적/생리학적 작용

Acetylcholinesterase (AChE) exhibits its activity against acetylcholine in the neuromuscular junctions and synaptic cleft of cholinergic neurons. It participates in the nitric oxide signaling pathway.
Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H2O to choline + acetic acid.

주의사항

For dilute solutions (<1 mg/ml), add 1 mg/ml BSA to stabilize. Solutions containing at least 1 mg protein/ml in dilute neutral phosphate buffer are stable at least 6 months refrigerated.

단위 정의

One unit will hydrolyze 1.0 μmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C.

물리적 형태

Lyophilized powder containing Tris buffer salts

분석 메모

The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Daniela Pereira et al.
Marine drugs, 19(1) (2020-12-31)
Over the last decades, antifouling coatings containing biocidal compounds as active ingredients were used to prevent biofouling, and eco-friendly alternatives are needed. Previous research from our group showed that polymethoxylated chalcones and glycosylated flavones obtained by synthesis displayed antifouling activity
Silvia Olivera et al.
Molecular and cellular neurosciences, 23(1), 96-106 (2003-06-12)
Here we show that chronic application of low concentrations (0.01-0.05 U/ml) or a single application of 1-5 U/ml acetylcholinesterase (AChE) promotes the extension of neuronal processes, synapse formation, and alpha-amino-3-hydroxy-5-methylisoxazolepropionate receptor (AMPAR) surface expression in both embryonic and postnatal hippocampal
Daniel A S Kitagawa et al.
Journal of enzyme inhibition and medicinal chemistry, 36(1), 1370-1377 (2021-06-22)
Organophosphorus poisoning caused by some pesticides and nerve agents is a life-threating condition that must be swiftly addressed to avoid casualties. Despite the availability of medical countermeasures, the clinically available compounds lack a broad spectrum, are not effective towards all
Nagham Alatrash et al.
ChemMedChem, 12(13), 1055-1069 (2017-06-13)
Four mononuclear [(L-L)2 Ru(tatpp)]2+ and two dinuclear [(L-L)2 Ru(tatpp)Ru(L-L)2 ]4+ ruthenium(II) polypyridyl complexes (RPCs) containing the 9,11,20,22-tetraazatetrapyrido[3,2-a:2',3'-c:3'',2''-l:2''',3'''-n]pentacene (tatpp) ligand were synthesized, in which L-L is a chelating diamine ligand such as 2,2'-bipyridine (bpy), 1,10-phenanthroline (phen), 3,4,7,8-tetramethyl-1,10-phenanthroline (Me4 phen) or 4,7-diphenyl-1,10-phenanthroline
Neda Rafat et al.
Biosensors, 11(11) (2021-11-26)
A novel, integrated experimental and modeling framework was applied to an inhibition-based bi-enzyme (IBE) electrochemical biosensor to detect acetylcholinesterase (AChE) inhibitors that may trigger neurological diseases. The biosensor was fabricated by co-immobilizing AChE and tyrosinase (Tyr) on the gold working

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.