A8022

Bovine Serum Albumin

heat shock fraction, pH 5.2, ≥96%

• 동의어(들): Albumin bovine serum, BSA, Bovine albumin
• CAS Number: 9048-46-8
• EC Number: 232-936-2
• MDL number: MFCD00130384
• UNSPSC 코드: 12352202
• NACRES: NA.27

속성

• 생물학적 소스: bovine
• Quality Level: 300
• 분석: ≥96%
• 형태: lyophilized powder
• 분자량: ~66 kDa
• 정제법: heat shock fractionation
• 포장: poly bottle of
• 기원: USA origin
• 기술: ELISA: suitable; blocking: suitable; electrophoresis: suitable; western blot: suitable
• pH: 5.2
• solubility: water: soluble (40 mg/ml)
• UniProt 수납 번호: P02769
• 외래 활성: BT virus, none detected; VSV virus, none detected
• 저장 온도: 2-8°C
• 유전자 정보: bovine ... ALB(280717)

설명

일반 설명

Bovine serum albumin (BSA) is a serum protein with a calculated molecular weight of 66,430 Daltons BSA consists of three domains with two subdomains under each, and is a α-helical, globular and non-glycosylated protein with 17-disulfide bonds.

BSA acts as a carrier protein for biomolecules like as fatty acids, amino acids, and steroids.

애플리케이션

Bovine Serum Albumin has been used:

• with primary antibodies to prevent non-specific binding in immunohistochemistry of heart sections
• as a component of trypsin inhibitor solution
• as a component of sterile sorting buffer for immunocytochemistry of common lymphoid progenitor (CLP) cells

Although we have not specifically tested this product in the following applications, several publications have cited use of A8022 in various applications such as:

• Embryo culture (Dobrinsky, J.R. et al., Biol. Reprod., 55(5), 1069-1074 (1996))
• Oocyte culture medium (Jung, Y.J., and Cheon, Y.-P., Dev. Reprod., 18(2), 117-125 (2014))
• Western blotting (Chronopoulos, A. et al., Nat. Commun., 7:7, 12630 (doi: 10.1038/ncomms12630) (2016)

생화학적/생리학적 작용

Bovine Serum Albumin is used in many applications, such as the following:

• A transporter for drugs, hormones and fatty acids.
• As a blocking agent in enzyme linked immunosorbent assay (ELISA) for preventing non-specific binding of antigens and antibodies to microtiter plates
• BSA is a crucial component of cell culture media and favors embryonic stem cells (hESC) differentiation.
• BSA shares structural features with human serum albumin. Its high solubility, low cost and purity and interaction with surfactants make it useful in the cosmetic and pharmaceutical industries.

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

제조 메모

Prepared using heat shock fractionation

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)