A4503

Bovine Serum Albumin

cold ethanol fraction, pH 5.2, ≥96%

• 동의어(들): Albumin bovine serum, BSA, Bovine albumin
• CAS Number: 9048-46-8
• EC Number: 232-936-2
• MDL number: MFCD00130384
• UNSPSC 코드: 12352202
• NACRES: NA.27

속성

• 생물학적 소스: bovine
• 분석: ≥96%
• 양식: lyophilized powder
• 분자량: ~66 kDa
• 정제법: cold ethanol fractionation
• 기원: USA origin
• 기술: ELISA: suitable; blood typing: suitable; microbiological culture: suitable
• 불순물: BT and VSV virus, tested
• 손실: ≤5%
• pH: 5.2
• solubility: water: soluble (40 mg/ml)
• UniProt 수납 번호: P02769
• 외래 활성: BT Virus, none detected; VSV Virus, none detected
• 저장 온도: 2-8°C
• 유전자 정보: bovine ... ALB(280717)

설명

일반 설명

Bovine serum albumin is a model protein in various studies and is made up of 583 amino acids. It is a water-soluble protein with a molecular weight of 66.4kDa. Six α-helices form three homologous domains of BSA. Depending on pH, it undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating.

애플리케이션

• Bovine Serum Albumin has been used in solution prepared for the isolation of heart mitochondria.
• It has been used in the cytotoxicity assay buffer.
• It has been used for the induction of protein overload nephropathy in rats.

생화학적/생리학적 작용

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

제조 메모

Often referred to as Cohn fraction V; this product is prepared by a modified method of the Cohn cold ethanol fractionation method.

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)