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Merck
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Key Documents

A3059

Sigma-Aldrich

Bovine Serum Albumin

heat shock fraction, protease free, essentially globulin free, pH 7, ≥98%

동의어(들):

Albumin bovine serum, BSA, Bovine albumin

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC 코드:
12352202
NACRES:
NA.27

생물학적 소스

bovine

Quality Level

분석

≥98%

형태

lyophilized powder

분자량

~66 kDa

정제법

heat shock fractionation

포장

poly bottle of

기원

USA origin

기술

ELISA: suitable
western blot: suitable

불순물

essentially globulin free
protease, none detected

손실

≤5%

pH

7

solubility

water: soluble (40 mg/ml)

UniProt 수납 번호

외래 활성

BT Virus, none detected
VSV Virus, none detected

저장 온도

2-8°C

유전자 정보

bovine ... ALB(280717)

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

Bovine serum albumin (BSA) has an important role in the regulation of blood colloidal osmotic pressure. BSA is the major protein in bovine serum, and is composed of 583 amino acids with a calculated molecular weight of 66,430 Daltons. BSA is used in many different applications and fields, such as:
  • Protein quantitation (as a standard)
  • Immunochemistry (as a blocking agent)
  • Cell culture (as a nutrient)

This particular BSA is specifically evaluated and tested to have very low protease content / for protease-free status. Protease-free BSA may be used in applications like:
  • Enzyme assays
  • Protein-based assays
  • Protease-sensitive techniques that include:
a) Enzyme immunoassay (EIA)
b) Nucleic acid hybridization
c) Radioimmunoassay (RIA)

애플리케이션

Bovine Serum Albumin has been used in luminescence assays.

생화학적/생리학적 작용

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

특징 및 장점

  • Heat shock fractionated
  • Protease-free
  • Essentially globulin-free

제조 메모

Prepared using heat shock fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


시험 성적서(COA)

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