콘텐츠로 건너뛰기
Merck
모든 사진(4)

주요 문서

A3803

Sigma-Aldrich

Bovine Serum Albumin

heat shock fraction, lyophilized powder, essentially fatty acid free, ≥98% (agarose gel electrophoresis)

동의어(들):

Albumin bovine serum, BSA, Bovine albumin

로그인조직 및 계약 가격 보기


About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC 코드:
12352202
NACRES:
NA.25

생물학적 소스

bovine

Quality Level

분석

≥98% (agarose gel electrophoresis)

양식

lyophilized powder

분자량

~66 kDa

정제법

heat shock fractionation

포장

poly bottle of

기원

USA origin

기술

cell culture | mammalian: suitable

불순물

≤0.005% Fatty acids

손실

≤5.0%, Loss on drying

pH

7

solubility

water: soluble (40 mg/ml)

적합성

suitable for ELISA applications (as diluent)

UniProt 수납 번호

외래 활성

BT Virus, none detected
VSV Virus, none detected

저장 온도

2-8°C

유전자 정보

bovine ... ALB(280717)

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

Bovine serum albumin (BSA) is a 66.4 kDa, water-soluble protein composed of 583 amino acids. It has a single polypeptide chain with three homologous domains formed by six α-helices. Depending on pH, it undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating. BSA has been used as a model protein in various applications, including immunodiagnostic procedures, cell culture media and clinical chemistry. Serum albumins facilitates drug disposition and efficacy by maintaining osmotic blood pressure.

애플리케이션

Bovine Serum Albumin has been used:
  • to prevent nonspecific adsorption of fibrin matrices to tissue culture plates
  • as a constituent in the preparation of albumin droplets
  • as a supplement in L-15 medium to increase spermatogonia number without enhancing the growth of somatic cells
  • as a standard protein to evaluate metal oxide adsorption isotherms

Among its many applications, BSA is a fatty acid (FA) carrier. Thus when BSA is used in applications like cell culture, it is important to exercise control over particular FA′s for cell culture, because specific fatty acids can affect different cell lines in a variable manner. In order to control fatty acid content in cell culture, fatty acid-free BSA is useful, for researchers to be able fatty acids specific to their cell lines. Fatty acid-free BSA also allows for optimal and maximum binding sites for the specific FA′s to use in cell culture. The use of FA-free BSA also addresses concerns about endogenous fatty acids that might be potentially present in non-FA-free BSA.

생화학적/생리학적 작용

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

특징 및 장점

  • Heat shock fractionated
  • Essentially fatty acid-free

제조 메모

Prepared using heat shock fractionation. Further purified to reduce fatty acid content.
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number

적합한 버전을 찾을 수 없으신가요?

특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Ninette Genster et al.
Scientific reports, 7(1), 3852-3852 (2017-06-22)
Ficolins are a family of pattern recognition molecules that are capable of activating the lectin pathway of complement. A limited number of reports have demonstrated a protective role of ficolins in animal models of infection. In addition, an immune modulatory
Marie Christou-Kent et al.
EMBO molecular medicine, 10(5) (2018-04-18)
The genetic causes of oocyte meiotic deficiency (OMD), a form of primary infertility characterised by the production of immature oocytes, remain largely unexplored. Using whole exome sequencing, we found that 26% of a cohort of 23 subjects with OMD harboured
Ov D Slayden et al.
Contraception, 94(5), 521-526 (2016-10-25)
Intrauterine administration of polidocanol foam (PF) can create fallopian tube occlusion in nonhuman primates. The objective of this study was to determine if PF-induced tubal obstructions contain collagen in the extracellular matrix. We compared tissue samples of the intramural fallopian
Initial investigation of acoustic droplet vaporization for occlusion in canine kidney
Zhang M, et al.
Ultrasound in Medicine & Biology, 36(10), 1691-1703 (2010)
Improved in vitro culture conditions to enhance the survival, mitotic activity, and transplantability of rainbow trout type a spermatogonia
Shikina S and Yoshizaki G
Biology of Reproduction, 83(2), 268-276 (2010)

문서

Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.