A2934

Bovine Serum Albumin

lyophilized powder, essentially globulin free, low endotoxin, ≥98% (agarose gel electrophoresis)

• 동의어(들): Albumin bovine serum, BSA, Bovine albumin
• CAS Number: 9048-46-8
• EC Number: 232-936-2
• MDL number: MFCD00130384
• UNSPSC 코드: 12352202
• NACRES: NA.27

속성

• 생물학적 소스: bovine
• Quality Level: 200
• 분석: ≥98% (agarose gel electrophoresis)
• 형태: lyophilized powder
• 분자량: ~66 kDa
• 정제법: heat shock fractionation
• 포장: poly bottle of
• 기원: USA origin
• 기술: ELISA: suitable
• 불순물: essentially globulin free; ≤1 ng/mg endotoxin
• pH: 5.2
• solubility: water: soluble (40 mg/ml)
• UniProt 수납 번호: P02769
• 외래 활성: BT Virus, none detected; VSV Virus, none detected
• 저장 온도: 2-8°C
• 유전자 정보: bovine ... ALB(280717)

설명

일반 설명

Bovine Serum Albumin (BSA) is a globular non-glycosylated protein that encompasses structurally distinct domains (I, II, and III) with two sub-domains, A and B within each domain. Structurally, BSA is an α-helical protein with 17 cysteine residues.

애플리케이션

Bovine Serum Albumin has been used:

• as a component of the minimal liquid medium to culture Porphyromonas gingivalis strains
• as a standard protein to quantify microsomes homogenate using Bradford method
• as a blocking agent in enzyme-linked immunosorbent assay (ELISA) of fecal lactoferrin

생화학적/생리학적 작용

Bovine Serum Albumin (BSA) is widely used in drugs and antibody delivery. It also finds applications in nanoparticle preparations and is useful in targeting tumor tissues. BSA is non-immunogenic and non-toxic. It also shows binding with metal ions.

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

특징 및 장점

• Low endotoxin
• Essentially globulin-free

제조 메모

Prepared using heat shock fractionation

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)