A9085

Bovine Serum Albumin

lyophilized powder, essentially IgG free, ≥96% (agarose gel electrophoresis)

• 동의어(들): Albumin bovine serum, BSA, Bovine albumin
• CAS Number: 9048-46-8
• EC Number: 232-936-2
• MDL number: MFCD00130384
• UNSPSC 코드: 12352202
• NACRES: NA.25

속성

• 생물학적 소스: bovine
• Quality Level: 200
• 분석: ≥96% (agarose gel electrophoresis)
• 형태: lyophilized powder
• 분자량: ~66 kDa
• 정제법: heat shock fractionation
• 포장: poly bottle of
• 기원: USA origin
• 기술: flow cytometry: suitable; immunofluorescence: suitable
• 불순물: IgG free; ≤25 ng/mg protein IgG
• pH: 7
• solubility: water: soluble (40 mg/ml)
• UniProt 수납 번호: P02769
• 외래 활성: Blue tongue virus, none detected; Vesicular Statitis virus, none detected
• 저장 온도: 2-8°C
• 유전자 정보: bovine ... ALB(280717)

설명

일반 설명

Bovine serum albumin (BSA) is a 66 kDa protein consisting of three domains with two subdomains under each. It is a α-helical, globular and non-glycosylated protein. BSA belongs to the serum albumins family. It has 17-disulfide bonds.

애플리케이션

Bovine Serum Albumin has been used in:

• the washing of the rabbit tissue sections in immunofluorescence microscopy
• incubation of red blood cells (RBC) for flow cytometry analysis
• as a component of Hanks′ balanced salt solution with Ca²⁺ and Mg²⁺ (HBSS++) for eosin-5-maleimide staining and RBC washing in magnetic circular dichroism measurements

생화학적/생리학적 작용

Bovine Serum Albumin (BSA) is a transporter for drugs, hormones and fatty acids. It is used as a blocking agent in enzyme linked immunosorbent assay (ELISA) for preventing non-specific binding of antigens and antibodies to the microtiter plates. It is a crucial component of the cell culture media and favors embryonic stem cells (hESC) differentiation. BSA shares structural features with human serum albumin. Its high solubility, low cost and purity and interaction with surfactant is exploited in cosmetic and pharmaceutical industry.

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

특징 및 장점

• Essentially IgG free (immunoglobulin-free)
• Heat shock fractionated

제조 메모

Prepared using heat shock fractionation.

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)