추천 제품
생물학적 소스
bovine
Quality Level
분석
>95% (total protein)
양식
liquid
제조업체/상표
Chemicon®
기술
cell based assay: suitable
UniProt 수납 번호
배송 상태
dry ice
유전자 정보
bovine (calf) ... PRNP(281427)
일반 설명
Histidine-tagged full-length mature part of bovine PrP (25-244) is expressed in E. Coli BL21, solubilized from inclusion bodies in 6 M guanidine-HCl, and purified by Ni(II)-nitriloacetate agarose chromatography followed by reversed-phase HPLC (C4 column)
Prion diseases or transmissible spongiform encephalopathies are neurodegenerative diseases that affect both humans and animals (Prusiner 1998). All prion diseases share the same molecular pathogenic mechanism that involves conversion of normal cellular prion protein (PrPc) into a form that is insoluble in non ionic detergent and partially resistant to proteases (PrPSc) (Pan et al. 1993). Both PrPSc and PrPc are encoded within a single exon of a chromosomal gene as a protein of ~ 250 amino acids (Basler et al. 1986). Many mammalian PrPs have a 22 amino acid N-terminal signal sequence (Hope et al. 1986; Turk et al. 1988) and 23 amino acid C-terminal signal sequence encoding for attachment of a glycosylphosphatidylinositol anchor (Stahl et al. 1987, 1990). The mature protein of 209 amino acids contains one disulfide bond (Turk et al. 1988) and has two sites of asparagine-linked glycosylation (Endo et al. 1989; Oesch et al. 1995).
애플리케이션
Antigen in standard immunochemical detection of BSE.
Optimal working dilution must be determined by the end user.
Optimal working dilution must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
물리적 형태
Liquid in 10 mM sodium acetate buffer, pH 4.0, containing 0.01% sodium azide.
저장 및 안정성
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
분석 메모
The PrPc appears as a single band of about 27 kD by SDS-PAGE
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Purification and properties of the cellular and scrapie hamster prion proteins.
Turk, E, et al.
European Journal of Biochemistry, 176, 21-30 (1988)
Scrapie and cellular PrP isoforms are encoded by the same chromosomal gene.
Basler, K, et al.
Cell, 46, 417-428 (1986)
Prions.
Prusiner, S B
Proceedings of the National Academy of Sciences of the USA, 95, 13363-13383 (1998)
Identification of glycoinositol phospholipid linked and truncated forms of the scrapie prion protein.
Stahl, N, et al.
Biochemistry, 29, 8879-8884 (1990)
Scrapie prion protein contains a phosphatidylinositol glycolipid.
Stahl, N, et al.
Cell, 51, 229-240 (1987)
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