AB9748-I

Anti-14-3-3 Antibody (pan)

from rabbit, purified by affinity chromatography

• 동의어(들): 14-3-3 protein beta/alpha, KCIP-1, Protein 1054, Protein kinase C inhibitor protein 1, 14-3-3 protein epsilon, 14-3-3E, 14-3-3 protein eta, Protein AS1, 14-3-3 protein gamma, 14-3-3 protein theta, 14-3-3 protein T-cell, 14-3-3 protein tau, Protein HS1, 1
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: human
• 종 반응성(상동성에 의해 예측): porcine (based on 100% sequence homology), mouse (based on 100% sequence homology), Xenopus (based on 100% sequence homology), fish (based on 100% sequence homology), zebrafish (based on 100% sequence homology), rabbit (based on 100% sequence homology), chicken (based on 100% sequence homology), sheep (based on 100% sequence homology), bovine (based on 100% sequence homology), rat (based on 100% sequence homology)
• 기술: immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• NCBI 수납 번호: NP_003395; NP_003396; NP_003397; NP_006752; NP_006817; NP_036611
• UniProt 수납 번호: P27348; P31946; P61981; P62258; P63104; Q04917
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... YWHAB(7529) , YWHAE(7531) , YWHAG(7532) , YWHAH(7533) , YWHAQ(10971) , YWHAZ(7534)

설명

일반 설명

14-3-3 protein beta/alpha (KCIP-1, Protein 1054, Protein kinase C inhibitor protein 1), epsilon (14-3-3E), eta (Protein AS1), gamma (KCIP-1, Protein kinase C inhibitor protein 1), theta (14-3-3 protein T-cell, 14-3-3 protein tau, Protein HS1), zeta/delta (KCIP-1, Protein kinase C inhibitor protein 1) are encoded by the YWHAB, YWHAE, YWHAH, YWHAG, YWHAQ, YWHAZ gene (Gene ID 7529, 7531, 7533, 7532, 10971, 7534, respectively) in human. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers, thus rendering 14-3-3 as a key multifunctional regulatory molecule. 14-3-3 isomers have been implicated in several neurological disorders: for example isoforms gamma, epsilon and beta are expressed in the cerebrospinal fluid of Creutzfeldt-Jakob patients, but not patients suffering from other neurological conditions such as Alzheimer′s. The 14-3-3 pan antibody is one of a panel of antibodies that were generated against 14-3-3 isoforms and have demonstrated their potential for use as an ELISA-based diagnostic tool. These antibodies will allow the research to investigate the role of this protein in key cellular processes and neurological disorders such as Creutzfeldt-Jakob, Alzheimer′s and Parkinson′s disease.

특이성

This antibody targets the N-terminal end sequences of 14-3-3 alpha/beta (YWHAB), gamma (YWHAG), zeta/delta (YWHAZ), epsilon (YWHAE), eta (YWHAH or YWHA1), and theta (YWHAQ).

면역원

A mixture of KLH-conjugated linear peptides corresponding the the N-terminal end sequences of human 14-3-3 alpha/beta (YWHAB), gamma (YWHAG), zeta/delta (YWHAZ), epsilon (YWHAE), eta (YWHAH or YWHA1), and theta (YWHAQ).

애플리케이션

Anti-14-3-3 pan, Cat. No. AB9748-I, is a highly specific rabbit polyclonal antibody, that targets 14-3-3 protein and has been tested in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting and .

Immunohistochemistry Analysis: A 1:5,000 dilution from a representative lot detected 14-3-3 immunoreactivity in human prostate cancer, cerebellum, and tonsil tissue sections.

Immunoprecipitation Analysis: 5 µg from a representative lot immunoprecipitated 14-3-3 from 500 µg of A431 cell lysate.

Research Category
Neuroscience

품질

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected 14-3-3 in 10 µg of A431 cell lysate.

표적 설명

~28 kDa observed. 28.08/27.85 kDa (human isoform Long/Short; YWHAB), 27.75 kDa (human YWHAZ), 29.17 kDa (human YWHAE), 28.22 kDa (human YWHAH or YWHA1), 28.30 kDa (human YWHAG), 27.76 kDa (human YWHAQ) calculated. Uncharacterized bands may be observed in some lysate(s).

결합

Replaces: AB9748

물리적 형태

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable