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Merck
모든 사진(3)

주요 문서

06-549

Sigma-Aldrich

Anti-c-Myc Tag Antibody

UPSTATE®, rabbit polyclonal

동의어(들):

Transcription factor p64, avian myelocytomatosis viral oncogene homolog, myc proto-oncogene protein, v-myc avian myelocytomatosis viral oncogene homolog, v-myc myelocytomatosis viral oncogene homolog (avian)

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.43

제품명

Anti-Myc Tag Antibody, Upstate®, from rabbit

생물학적 소스

rabbit

Quality Level

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

polyclonal

종 반응성

human

포장

antibody small pack of 25 μg

제조업체/상표

Upstate®

기술

immunocytochemistry: suitable
western blot: suitable

동형

IgG

NCBI 수납 번호

UniProt 수납 번호

배송 상태

dry ice

타겟 번역 후 변형

unmodified

일반 설명

Myc proto-oncogene protein (UniProt: P01106; also known as Class E basic helix-loop-helix protein 39 (bHLHe39), Proto-oncogene c-Myc, Transcription factor p64) is encoded by the MYC (also known as BHLHE39) gene (Gene ID: 4609) in human. The Myc-tag is a short peptide sequence derived from the c-Myc protein. It is commonly used as an epitope tag in molecular biology. It is composed of 10 amino acids (EQKLISEEDL) and is fused to proteins of interest to facilitate their detection and purification. The Myc-tag functions as a marker that can be recognized by specific antibodies, allowing researchers to track the expression, localization, and interaction of tagged proteins in various experimental contexts.

특이성

Other species cross-reactivity not tested.
This rabbit polyclonal antibody detects human Myc and Myc-tagged recombinant proteins.

면역원

Epitope: a.a. 409-420
Synthetic peptide corresponding to residues 409-420 of human Myc (MEQKLISEEDLN).

애플리케이션

Anti-Myc Tag, Cat. No. 06-549, is a rabbit polyclonal antibody that detects Myc-tagged proteins and is tested for use in Western Blotting and Immunocytochemistry.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Tested Applications Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected Myc-tagged PP2A A subunit in NIH/3T3 cells transfected with Myc-tagged PP2A A subunit. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

품질

Evaluated by Western Blotting in NIH/3T3 cells transfected with a Myc-tagged PP2A A subunit. Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected the Myc-tag in NIH/3T3 cells transfected with Myc-tagged PP2A A subunit.

표적 설명

Predicted molecular weight for human Myc: 49 kDa Obeserved molecular weight may vary.

물리적 형태

Format: Purified
Protein A chromatography
Purified rabbit in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide. Frozen solution.

저장 및 안정성

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

분석 메모

Control
Positive control for human Myc: widespread expression including A431 cell lysate, ovarian cancer cell lysate, or breast carcinoma tissue. Myc fusion protein expressed in cells.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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