F7425

ANTI-FLAG^® antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• 동의어(들): Anti-ddddk, Anti-dykddddk
• MDL number: MFCD02262912
• UNSPSC 코드: 12352203
• NACRES: NA.32

속성

• 생물학적 소스: rabbit
• 결합: unconjugated
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 양식: buffered aqueous solution
• 종 반응성: all
• 농도: ~0.8 mg/mL
• 기술: dot blot: 1-2.5 μg/mL; immunoprecipitation (IP): 4-8 μg using amino terminal FLAG-BAP fusion protein from E. coli crude lysate; indirect immunofluorescence: 5-10 μg/mL using 293T cells transfected with a plasmid encoding FLAG-JNK; western blot (chemiluminescent): 1-2.5 μg/mL using an E. coli periplasmic extract expressing an N-terminal FLAG fusion protein
• 동형: IgG
• 면역원 서열: DYKDDDDK
• 배송 상태: dry ice
• 저장 온도: −20°C

설명

일반 설명

The FLAG epitope, is an eight amino acid protein with an enterokinase-cleavage site.

The rabbit Anti-FLAG polyclonal affinity antibody ANTI-FLAG recognizes the FLAG epitope located on FLAG fusion proteins. This antibody reacts with N-terminal, N-terminal-Met, and C-terminal FLAG fusion proteins.

면역원

FLAG; peptide sequence DYKDDDDK

애플리케이션

The antibody recognizes the FLAG epitope located on FLAG-tagged fusion proteins at the N-terminus or C-terminus, applying dot blot, immunoblotting, immunoprecipitation and immunocytochemistry assays.
Learn more product details in our FLAG® application portal."

생화학적/생리학적 작용

FLAG epitope plays a crucial role in immunoaffinity purification of fusion proteins.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

제조 메모

Purified by affinity chromatography on a column bearing the immunizing peptide.

기타 정보

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

법적 정보

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable