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Merck
모든 사진(4)

주요 문서

F7425

Millipore

ANTI-FLAG® antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

동의어(들):

Anti-ddddk, Anti-dykddddk

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About This Item

MDL number:
UNSPSC 코드:
12352203
NACRES:
NA.32

생물학적 소스

rabbit

Quality Level

결합

unconjugated

항체 형태

affinity isolated antibody

항체 생산 유형

primary antibodies

클론

polyclonal

양식

buffered aqueous solution

종 반응성

all

농도

~0.8 mg/mL

기술

dot blot: 1-2.5 μg/mL
immunoprecipitation (IP): 4-8 μg using amino terminal FLAG-BAP fusion protein from E. coli crude lysate
indirect immunofluorescence: 5-10 μg/mL using 293T cells transfected with a plasmid encoding FLAG-JNK
western blot (chemiluminescent): 1-2.5 μg/mL using an E. coli periplasmic extract expressing an N-terminal FLAG fusion protein

동형

IgG

면역원 서열

DYKDDDDK

배송 상태

dry ice

저장 온도

−20°C

일반 설명

The FLAG epitope, is an eight amino acid protein with an enterokinase-cleavage site.
The rabbit Anti-FLAG polyclonal affinity antibody ANTI-FLAG recognizes the FLAG epitope located on FLAG fusion proteins. This antibody reacts with N-terminal, N-terminal-Met, and C-terminal FLAG fusion proteins.

면역원

FLAG; peptide sequence DYKDDDDK

애플리케이션

The antibody recognizes the FLAG epitope located on FLAG-tagged fusion proteins at the N-terminus or C-terminus, applying dot blot, immunoblotting, immunoprecipitation and immunocytochemistry assays.
Learn more product details in our FLAG® application portal."

생화학적/생리학적 작용

FLAG epitope plays a crucial role in immunoaffinity purification of fusion proteins.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

제조 메모

Purified by affinity chromatography on a column bearing the immunizing peptide.

법적 정보

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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