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Merck
모든 사진(3)

주요 문서

M4439

Sigma-Aldrich

Anti-c-Myc antibody, Mouse monoclonal

clone 9E10, purified from hybridoma cell culture

동의어(들):

Anti-c-Myc

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About This Item

UNSPSC 코드:
12352203
NACRES:
NA.56

생물학적 소스

mouse

결합

unconjugated

항체 형태

purified from hybridoma cell culture

항체 생산 유형

primary antibodies

클론

9E10, monoclonal

양식

buffered aqueous solution

분자량

62 kDa

종 반응성

human

농도

~2 mg/mL

기술

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1:5000 using c-myc tagged fusion protein

UniProt 수납 번호

배송 상태

dry ice

저장 온도

−20°C

유전자 정보

human ... MYC(4609)

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

The mouse Anti-c-MYC monoclonal antibody recognizes an epitope located within a sequence (EQKLISEEDL) of the product of the human oncogene c-myc.
c-Myc (v-myc avian myelocytomatosis viral oncogene homolog) is a transcription factor, which is coded by MYC gene. It was first described as a homolog of an avian retroviral oncogene. It belongs to the family of transcription factors which are characterized by basic region helix-loop-helix/leucine zipper. Along with N-Myc and L-Myc, it forms the MYC proto-oncogene family. It forms a heterodimer with MAX, and this heterodimer binds to the E-boxes/CACGTG sequence motif on DNA. This gene is localized to human chromosome 8q24.

면역원

synthetic peptide of the human p62c-Myc protein.

애플리케이션

Monoclonal Anti-c-Myc antibody produced in mouse is suitable for immunoprecipitation, immunoblotting, ELISA, immunofluorescence, microarray and electron microscopy. It has also been used in ChIP (Chromatin Immunoprecipitation).

생화학적/생리학적 작용

v-myc avian myelocytomatosis viral oncogene homolog belongs to MYC family. When overexpressed these proteins can cause DNA double-stand breaks (DSBs) and genome instability. This transcription factor MYC helps in regulating long noncoding RNAs (lncRNAs) which has been implicated in cancer cell proliferation and tumorigenesis. Its overexpression is also associated with various cancers, and in particular B cell lymphomas. Increased expression of c-MYC has a negative effect on the ability of B cell lymphomas to functionally present Ags/peptides to CD4+ T cells. This protein exerts suppressive effects at several critical checkpoints in Ag presentation. It is associated with Burkitt lymphoma and a group of diffuse large B-cell lymphoma (DLBCL).

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

제조 메모

Working dilution: 1:5000

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Željka Škunca et al.
Acta medica Croatica : casopis Hravatske akademije medicinskih znanosti, 68(3), 299-305 (2015-05-29)
Diffuse large B-cell lymphoma (DLBCL) is classified as lymphoma and various entities using the gene expression of proteins are classified into three groups. The aim of this study was to clarify the clinical, biological, immunophenotypic and cytogenetic features of DLBCL
Taewan Kim et al.
Journal of the National Cancer Institute, 107(4), doi:10-doi:10 (2015-02-11)
The functions of long noncoding RNAs (lncRNAs) have been identified in several cancers, but the roles of lncRNAs in colorectal cancer (CRC) are less well understood. The transcription factor MYC is known to regulate lncRNAs and has been implicated in
Susanna Ambrosio et al.
Mutation research, 774, 6-13 (2015-03-17)
Although it is established that when overexpressed, the MYC family proteins can cause DNA double-stand breaks (DSBs) and genome instability, the mechanisms involved remain unclear. MYC induced genetic instability may result from increased DNA damage and/or reduced DNA repair. Here
Mirjana Macvanin et al.
Journal of bacteriology, 194(22), 6046-6055 (2012-09-04)
Some unidentified RNA molecules, together with the nucleoid protein HU, were suggested to be involved in the nucleoid structure of Escherichia coli. HU is a conserved protein known for its role in binding to DNA and maintaining negative supercoils in
Jun-Sub Kim et al.
Molecular biology of the cell, 20(11), 2650-2660 (2009-04-03)
Cellular stimuli generate reactive oxygen species (ROS) via the local action of NADPH oxidases (Nox) to modulate cytoskeletal organization and cell migration through unknown mechanisms. Cofilin is a major regulator of cellular actin dynamics whose activity is controlled by phosphorylation/dephosphorylation

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