タンパク質のHPLC分析、TSKgel^® Phenyl-5PW

column

TSKgel Phenyl-5PW, 7.5 cm × 7.5 mm I.D., 10 μm particles (807573)

mobile phase

0.1 M phosphate buffer, pH 7.0 plus ammonium sulfate, 1.8 M to 0 M in 60 min

flow rate

1 mL/min

detector

UV, 280 nm