HPLC Analysis of Proline Enantiomers on Astec® CHIROBIOTIC® T (Formic Acid Mobile Phase Additive)
CONDITIONS
column
CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)
mobile phase
[A] water; [B] methanol; [C] formic acid; (40:60:0.02, A:B:C)
flow rate
1 mL/min
column temp.
25 °C
detector
UV, 205 nm
injection
10 μL
sample
200 μg /mL in 50:50 water:methanol
詳細
法的情報
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC