HPLC Analysis of Paclitaxel and Related Compounds on Ascentis® Express F5
CONDITIONS
column
Ascentis Express F5, 15 cm x 4.6 mm I.D., 5 μm particles (50631-U)
mobile phase
[A] water; [B] acetonitrile; (60:40, A:B)
flow rate
1.5 mL/min
pressure
2727 psi (188 bar)
column temp.
30 °C
detector
UV, 227 nm
injection
5 μL
sample
taxols 25 μg/mL in 70:15:15, water:methanol:acetonitrile
詳細
アナリシスノート
An efficient reverse phase HPLC method for the HPLC analysis of of Paclitaxel (Taxol) and related compounds is developed using the Ascentis® Express F5 5μm Fused Core HPLC column. Paclitaxel is a natural product with antitumor activity. TAXOL (paclitaxel) is obtained via a semi-synthetic process from Taxus baccata.
The developed method can be easily used for the routine quality control of bulk and Parenterals dosage form of Paclitaxel within a short analysis time. This method offers the advantages of higher efficies, reduced backpressure and shorter run times over a traditionally run methods over 5 μm totally porous particle columns.
法的情報
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany