HPLCによるメチオニンエナンチオマーの分析、CHIROBIOTIC^® T2

column

CHIROBIOTIC T2, 25 cm x 4.6 mm I.D., 5 μm particles (16024AST)

mobile phase

[A] water; [B] acetonitrile; (25:75, A:B)

flow rate

1 mL/min

column temp.

25 °C

detector

UV, 220 nm

injection

1 μL

sample

1 mg/mL in methanol